Basic Vector Information
DH4-27 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
DH4-27 vector Sequence
LOCUS 40924_600 5088 bp DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector DH4-27, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5088) AUTHORS Huang DC, Holtz WJ, Maharbiz MM. TITLE A genetic bistable switch utilizing nonlinear protein degradation JOURNAL J Biol Eng 6 (1), 9 (2012) PUBMED 22776405 REFERENCE 2 (bases 1 to 5088) AUTHORS Huang DC, Holtz WJ, Maharbiz MM. TITLE Direct Submission JOURNAL Submitted (10-JUN-2012) Electrical Engineering and Computer Sciences, University of California, Berkeley, 656 Sutardja Dai Hall, Berkeley, CA 94720, USA REFERENCE 3 (bases 1 to 5088) TITLE Direct Submission REFERENCE 4 (bases 1 to 5088) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J Biol Eng"; date: "2012"; volume: "6"; issue: "1"; pages: "9" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (10-JUN-2012) Electrical Engineering and Computer Sciences, University of California, Berkeley, 656 Sutardja Dai Hall, Berkeley, CA 94720, USA" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT ##Assembly-Data-START## Assembly Method :: GENtle v. 1.9.4 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5088 /mol_type="other DNA" /organism="synthetic DNA construct" regulatory 1..82 /label=pRM /note="pRM" /regulatory_class="promoter" misc_feature 9..26 /label=oR1 /note="oR1" misc_feature 33..50 /label=oR2 /note="oR2" misc_feature 59..65 /label=oR3mut /note="oR3mut" 5'UTR 89..123 CDS 124..834 /label=lambda repressor /note="phage lambda repressor" misc_feature 835..915 /gene="cI with mf degradation tag" /label=mf-ssrA tag (LVA) /note="mf-ssrA tag (LVA)" 5'UTR 928..968 CDS 969..1589 /label=TetR /note="tetracycline repressor TetR" CDS 1590..1622 /label=ssrA tag (LVA) /note="C-terminal peptide that mediates degradation in bacteria through the ClpXP and ClpAP proteases (McGinness et al., 2006)" terminator 1661..1732 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 1748..1775 /label=T7Te terminator /note="phage T7 early transcription terminator" CDS complement(2309..3256) /label=Rep101 /note="RepA protein needed for replication with the pSC101 origin" rep_origin complement(3304..3526) /direction=LEFT /label=pSC101 ori /note="low-copy replication origin that requires the Rep101 protein" terminator complement(4018..4112) /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(4146..4937) /label=NeoR/KanR /note="aminoglycoside phosphotransferase"
This page is informational only.