CS108 vector (V010030)

Basic Vector Information

      • Vector Name:
      • CS108
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 4149 bp
      • Type:
      • Expression vector
      • Source/Author:
      • Khokha MK, Hsu D, Baker JC, Harland RM.

CS108 vector Vector Map

CS1084149 bp60012001800240030003600SP6 promoterT7 promoterSV40 poly(A) signalT3 promoterM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoterf1 oriCMV IE94 promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

CS108 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_545        4149 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector CS108, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Khokha MK, Hsu D, Baker JC, Harland RM.
  TITLE     Vectors for Expression Cloning in Xenopus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Khokha MK, Hsu D, Baker JC, Harland RM.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAY-2006) MCB, UC-Berkeley, 142 LSA, Berkeley, CA 
            94720-3200, USA
REFERENCE   3  (bases 1 to 4149)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4149)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-MAY-2006) MCB, UC-Berkeley, 142 LSA, Berkeley, CA 94720-3200, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4149
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        complement(167..185)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    complement(190..324)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(441..459)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(480..496)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(504..520)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(528..558)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(573..594)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(882..1470)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1644..2501)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2502..2606)
                     /label=AmpR promoter
     rep_origin      complement(2632..3087)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3165..4149
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus
                     major immediate early transcription unit IE94"

This page is informational only.