Basic Vector Information
CS108 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
CS108 vector Sequence
LOCUS 40924_545 4149 bp DNA circular SYN 17-DEC-2018 DEFINITION Expression vector CS108, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4149) AUTHORS Khokha MK, Hsu D, Baker JC, Harland RM. TITLE Vectors for Expression Cloning in Xenopus JOURNAL Unpublished REFERENCE 2 (bases 1 to 4149) AUTHORS Khokha MK, Hsu D, Baker JC, Harland RM. TITLE Direct Submission JOURNAL Submitted (25-MAY-2006) MCB, UC-Berkeley, 142 LSA, Berkeley, CA 94720-3200, USA REFERENCE 3 (bases 1 to 4149) TITLE Direct Submission REFERENCE 4 (bases 1 to 4149) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (25-MAY-2006) MCB, UC-Berkeley, 142 LSA, Berkeley, CA 94720-3200, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..4149 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 35..53 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" promoter complement(167..185) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" polyA_signal complement(190..324) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" promoter complement(441..459) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(480..496) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(504..520) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(528..558) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(573..594) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(882..1470) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1644..2501) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(2502..2606) /label=AmpR promoter rep_origin complement(2632..3087) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 3165..4149 /label=CMV IE94 promoter /note="enhancer/promoter region of simian cytomegalovirus major immediate early transcription unit IE94"
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