Cre-excised pCOIN DV vector (V010033)

Basic Vector Information

      • Vector Name:
      • Cre-excised pCOIN DV
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 6228 bp
      • Type:
      • Mammalian vector
      • Source/Author:
      • De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R.

Cre-excised pCOIN DV vector Vector Map

Cre-excised pCOIN DV6228 bp30060090012001500180021002400270030003300360039004200450048005100540057006000chicken beta-globin 5'HS4 insulator core sequencechicken beta-globin 5'HS4 insulator core sequencePGK promotervertebrate consensus sequence for strong initiation of translation (Kozak, 1987)FRT mutantM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoterM13 fwdFRTAd SASK primerloxPM13 fwdattR4ccdBCmRlac UV5 promoterattR3M13 revbGH poly(A) signal

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

Cre-excised pCOIN DV vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_515        6228 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian vector Cre-excised pCOIN DV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6228)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6228)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6228)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    35..277
                     /label=chicken beta-globin 5'HS4 insulator core sequence
                     /note="chicken beta-globin 5'HS4 insulator core sequence"
     misc_feature    278..520
                     /label=chicken beta-globin 5'HS4 insulator core sequence
                     /note="chicken beta-globin 5'HS4 insulator core sequence"
     promoter        546..1045
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     regulatory      1059..1068
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1070..1117
                     /label=FRT mutant
                     /note="FRT mutant"
     primer_bind     complement(1162..1178)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1186..1202)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1210..1240)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1255..1276)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1564..2152)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2326..3183)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3184..3288)
                     /label=AmpR promoter
     primer_bind     3762..3778
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3801..3848
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    3865..3968
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     primer_bind     4001..4017
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4031..4064
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     4139..4155
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4162..4286
                     /label=attR4
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(4330..4632)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(4977..5633)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(5687..5717)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(5743..5866)
                     /label=attR3
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5894..5910)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    5959..6183
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"

This page is informational only.