CIpNAT plasmid CIpNat vector (V010036)

Basic Vector Information

      • Vector Name:
      • CIpNAT plasmid CIpNat
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 7822 bp
      • Type:
      • Integrating vector
      • Source/Author:
      • Shahana S, Childers DS, Ballou ER, Bohovych I, Odds FC, Gow NA, Brown AJ.

CIpNAT plasmid CIpNat vector Vector Map

CIpNAT plasmid CIpNat7822 bp30060090012001500180021002400270030003300360039004200450048005100540057006000630066006900720075007800f1 oriM13 fwdT7 promoterRPS1 targeting sequenceSK primerFRT (minimal)TEF promoterNrsRTEF terminatorCandida albicans ACT1 terminator (Staib et al., 1999)FLPCandida albicans SAP2 promoter (Staib et al., 1999)SK primerFLP recombination target sequence (FRT) (Staib et al., 1999)T3 promoterM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

CIpNAT plasmid CIpNat vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_485        7822 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Integrating vector CIpNAT plasmid CIpNat, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7822)
  AUTHORS   Shahana S, Childers DS, Ballou ER, Bohovych I, Odds FC, Gow NA, 
            Brown AJ.
  TITLE     New Clox Systems for Rapid and Efficient Gene Disruption in Candida 
            albicans
  JOURNAL   PLoS ONE 9 (6), E100390 (2014)
  PUBMED    24940603
REFERENCE   2  (bases 1 to 7822)
  AUTHORS   Shahana S, Childers DS, Ballou ER, Bohovych I, Odds FC, Gow NA, 
            Brown AJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2014) School of Medical Sciences, University of 
            Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen AB25
            2ZD, UK
REFERENCE   3  (bases 1 to 7822)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7822)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2014"; volume: "9"; issue: "6"; pages: "E100390"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-JAN-2014) School of Medical Sciences, University of Aberdeen, 
            Institute of Medical Sciences, Foresterhill, Aberdeen AB25 2ZD, UK"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..462
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_recomb     1040..1513
                     /label=RPS1 targeting sequence
                     /note="RPS1 targeting sequence"
     primer_bind     complement(1511..1527)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1544..1560
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1562..1595
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     promoter        1636..1979
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1999..2562
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     terminator      2580..2777
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     regulatory      complement(2810..3206)
                     /note="Candida albicans ACT1 terminator (Staib et al.,
                     1999)"
                     /regulatory_class="terminator"
     CDS             complement(3207..4475)
                     /label=FLP
                     /note="site-specific recombinase"
     regulatory      complement(4500..5542)
                     /gene="SAP2"
                     /note="Candida albicans SAP2 promoter (Staib et al., 1999)"
                     /regulatory_class="promoter"
     gene            complement(4500..5542)
                     /gene="SAP2"
                     /label=SAP2
     primer_bind     5554..5570
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(5572..5605)
                     /note="FLP recombination target sequence (FRT) (Staib et
                     al., 1999)"
     protein_bind    5572..5605
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     promoter        complement(5638..5656)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5677..5693)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5701..5717)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5724..5754)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5769..5790)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6078..6666)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6840..7697)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7698..7802)
                     /label=AmpR promoter

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