Basic Vector Information
CIp10 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
CIp10 vector Sequence
LOCUS 40924_470 5198 bp DNA circular SYN 17-DEC-2018
DEFINITION Integrating vector CIp10, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5198)
AUTHORS Murad AM, Lee PR, Broadbent ID, Barelle CJ, Brown AJ.
TITLE CIp10, an efficient and convenient integrating vector for Candida
albicans
JOURNAL Yeast 16 (4), 325-327 (2000)
PUBMED 10669870
REFERENCE 2 (bases 1 to 5198)
AUTHORS Abdul Murad AM, Lee PR, Broadbent ID, Brown AJP.
TITLE Direct Submission
JOURNAL Submitted (31-AUG-1999) Molecular and Cell Biology, University of
Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen AB25
2ZD, UK
REFERENCE 3 (bases 1 to 5198)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 5198)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Yeast";
date: "2000"; volume: "16"; issue: "4"; pages: "325-327"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(31-AUG-1999) Molecular and Cell Biology, University of Aberdeen,
Institute of Medical Sciences, Foresterhill, Aberdeen AB25 2ZD, UK"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5198
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(3..458)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 600..616
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 626..644
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1124..1933
/gene="URA3"
/label=URA3
/note="Orotidine 5'-phosphate decarboxylase from Candida
albicans (strain SC5314 / ATCC MYA-2876). Accession#:
P13649"
primer_bind 2060..2076
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 2585..2590
/label=StuI site for targeted integration
/note="StuI site for targeted integration"
misc_feature 2602..2607
/label=NcoI site for targeted integration
/note="NcoI site for targeted integration"
primer_bind complement(2964..2980)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(3010..3028)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(3049..3065)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(3073..3089)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3097..3127)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(3142..3163)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(3451..4039)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4213..5070)
/label=AmpR
/note="beta-lactamase"
promoter complement(5071..5175)
/label=AmpR promoter
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