CIp10 vector (V010039)

Basic Vector Information

      • Vector Name:
      • CIp10
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 5198 bp
      • Type:
      • Integrating vector
      • Source/Author:
      • Murad AM, Lee PR, Broadbent ID, Barelle CJ, Brown AJ.

CIp10 vector Vector Map

CIp105198 bp6001200180024003000360042004800f1 oriM13 fwdT7 promoterURA3SK primerKS primerT3 promoterM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

CIp10 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                5198 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Integrating vector CIp10, complete sequence.
ACCESSION   AF181970
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Murad AM, Lee PR, Broadbent ID, Barelle CJ, Brown AJ.
  TITLE     CIp10, an efficient and convenient integrating vector for Candida 
            albicans
  JOURNAL   Yeast 16 (4), 325-327 (2000)
  PUBMED    10669870
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   Abdul Murad AM, Lee PR, Broadbent ID, Brown AJP.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-1999) Molecular and Cell Biology, University of 
            Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen AB25
            2ZD, UK
REFERENCE   3  (bases 1 to 5198)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Integrating vector CIp10"
                     /lab_host="Candida albicans"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:104091"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            670..2060
                     /gene="URA3"
                     /label=URA3
     primer_bind     2060..2076
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            complement(2072..2938)
                     /gene="RP10"
                     /label=RP10
     misc_feature    2585..2590
                     /label=StuI site for targeted integration
                     /note="StuI site for targeted integration"
     misc_feature    2602..2607
                     /label=NcoI site for targeted integration
                     /note="NcoI site for targeted integration"
     misc_feature    2939..669
                     /label=pBluescript II KS+ plasmid backbone
                     /note="pBluescript II KS+ plasmid backbone"
     primer_bind     complement(2964..2980)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(3010..3028)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3049..3065)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3073..3089
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3097..3127)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3142..3163
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3451..4039)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4210..5070)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5071..5175)
                     /gene="bla"
                     /label=AmpR promoter

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