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pBlueBacHis2 C vector (V010073)

Basic Vector Information

pBlueBacHis2 A (4863 bp), pBlueBacHis2 B (4853 bp), and pBlueBacHis2 C (4861 bp) are baculovirus transfer vectors that contain the Xpress N-terminal tag for expression and purification of recombinant fusion proteins. The pBlueBacHis2 vector is provided in three different versions for simplified cloning of your gene in-frame with the Xpress tag. The vectors contain sequences homologous to the lacZ gene and ORF1629 sequences in Bac-N-Blue linear DNA, allowing production of blue, recombinant plaques. Expression of your recombinant fusion protein is driven by the polyhedrin promoter.

Vector Name:
pBlueBacHis2 C
Antibiotic Resistance:
Ampicillin
Length:
4861 bp
Type:
Insect Cell Expression Vectors
Replication origin:
ori
Copy Number:
High copy number
Promoter:
polyhedrin
Cloning Method:
Enzyme digestion and ligation
5' Primer:
polyhedrin forward
Fusion Tag:
His

pBlueBacHis2 C vector Vector Map

pBlueBacHis2 C4861 bp6001200180024003000360042004800polyhedrin promoter6xHisT7 tag (gene 10 leader)Xpress(TM) tagcontains part of ORF1629AmpR promoterAmpRori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pBlueBacHis2 C vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_6721        4861 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4861)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..94
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             110..127
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             131..163
                     /codon_start=1
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
                     /translation="MASMTGGQQMG"
     CDS             167..190
                     /codon_start=1
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
                     /translation="DLYDDDDK"
     misc_recomb     265..970
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     promoter        1546..1650
                     /label=AmpR promoter
     CDS             1651..2508
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2682..3270
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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