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BBa_J96004 vector

Basic Vector Information

Basic Vector Information
Vector Name BBa_J96004 Antibiotic Resistance Ampicillin
Length 3478 bp Type Biobrick cloning vector
Source Constante M, Grunberg R, Isalan M.

BBa_J96004 vector Vector Map

BBa_J96004 vector Sequence

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LOCUS       Exported                3478 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Biobrick cloning vector BBa_J96004, complete sequence.
ACCESSION   JN204873
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Constante M, Grunberg R, Isalan M.
  TITLE     A biobrick library for cloning custom eukaryotic plasmids
  JOURNAL   PLoS ONE 6 (8), E23685 (2011)
  PUBMED    21901127
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Constante M, Grunberg R, Isalan M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JUL-2011) Systems Biology, CRG, C/ Dr. Aiguader, 88, 
            Barcelona, Barcelona 08003, Spain
REFERENCE   3  (bases 1 to 3478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 18, 2018 from SnapGene 4.1.8
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Biobrick cloning vector BBa_J96004"
                     /mol_type="other DNA"
                     /label=pSB1AT3 backbone
                     /note="pSB1AT3 backbone"
                     /db_xref="taxon:1084329"
     misc_feature    1..21
                     /label=Biobrick RFC23 format prefix
                     /note="Biobrick RFC23 format prefix"
     misc_feature    1..20
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     misc_feature    22..55
                     /label=MCS1c Biobrick sequence
                     /note="MCS1c Biobrick sequence"
     misc_feature    55..75
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     misc_feature    56..75
                     /label=Biobrick RFC23 format suffix
                     /note="Biobrick RFC23 format suffix"
     primer_bind     complement(211..230)
                     /note="VR reverse sequencing primer annealing site 
                     (BBa_G00102)"
     rep_origin      complement(317..929)
                     /direction=LEFT
                     /label=pMB1
                     /note="pMB1"
     rep_origin      complement(330..918)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1105..1133
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             1181..2371
                     /codon_start=1
                     /gene="tet"
                     /product="tetracyclin resistance protein"
                     /label=tet
                     /note="confers resistance to tetracyclin"
                     /protein_id="AEO27249.1"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     gene            1181..2371
                     /gene="tet"
                     /label=tet
     CDS             complement(2378..3238)
                     /codon_start=1
                     /gene="amp"
                     /product="ampicillin resistance protein"
                     /label=amp
                     /note="confers resistance to ampicillin"
                     /protein_id="AEO27250.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(2378..3238)
                     /gene="amp"
                     /label=amp
     promoter        complement(3239..3343)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3361..3380
                     /note="VF2 forward sequencing primer annealing site 
                     (BBa_G00100)"
     terminator      3433..3476
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"

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