Basic Vector Information
ARR6-LUC-NOS-At5g62920 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
ARR6-LUC-NOS-At5g62920 vector Sequence
LOCUS 40924_295 7129 bp DNA circular SYN 17-DEC-2018 DEFINITION Control vector ARR6-LUC-NOS-At5g62920, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7129) AUTHORS Hwang I, Sheen J. TITLE Two-component circuitry in Arabidopsis cytokinin signal transduction JOURNAL Nature 413 (6854), 383-389 (2001) PUBMED 11574878 REFERENCE 2 (bases 1 to 7129) AUTHORS Sheen J. TITLE Direct Submission JOURNAL Submitted (27-OCT-2006) Molecular Biology, Massachusetts General Hospital, 185 Cambridge St. Simches Research Building-CPZN7600, Boston, MA 02114, USA REFERENCE 3 (bases 1 to 7129) TITLE Direct Submission REFERENCE 4 (bases 1 to 7129) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nature"; date: "2001"; volume: "413"; issue: "6854"; pages: "383-389" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (27-OCT-2006) Molecular Biology, Massachusetts General Hospital, 185 Cambridge St. Simches Research Building-CPZN7600, Boston, MA 02114, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..7129 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind complement(15..31) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(39..55) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(63..93) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(108..129) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(417..1005) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1179..2036) /label=AmpR /note="beta-lactamase" promoter complement(2037..2141) /label=AmpR promoter primer_bind 2615..2631 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" regulatory 2667..5065 /label=ARR6 promoter /note="ARR6 promoter" /regulatory_class="promoter" regulatory 4996..5005 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" CDS 5066..6715 /label=luciferase /note="firefly luciferase" terminator 6873..7124 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal"
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