AND gate BCD-RFP vector (V010118)

LOCUS       40924_255        5021 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Boolean integrase logic vector AND gate BCD-RFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Courbet A, Endy D, Renard E, Molina F, Bonnet J.
  TITLE     Detection of pathological biomarkers in human clinical samples via 
            amplifying genetic switches and logic gates
  JOURNAL   Sci Transl Med 7 (289), 289RA83 (2015)
  PUBMED    26019219
REFERENCE   2  (bases 1 to 5021)
  AUTHORS   Courbet A, Molina F, Endy D, Bonnet J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2014) SysDiag CNRS UMR3145, CNRS, 1682 Rue de la 
            Valsiere, Montpellier, Herault 34184, France
REFERENCE   3  (bases 1 to 5021)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5021)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med"; date: "2015"; volume: "7"; issue: "289"; pages: "289RA83"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-JUL-2014) SysDiag CNRS UMR3145, CNRS, 1682 Rue de la Valsiere, 
            Montpellier, Herault 34184, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     regulatory      23..69
                     /label=P7
                     /note="P7"
                     /regulatory_class="promoter"
     misc_feature    76..128
                     /label=attB TP901-1
                     /note="attB TP901-1"
     terminator      137..208
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      224..251
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    258..307
                     /label=attP TP901-1
                     /note="attP TP901-1"
     misc_feature    314..363
                     /label=attB Bxb1
                     /note="attB Bxb1"
     regulatory      364..476
                     /label=j61048
                     /note="j61048"
                     /regulatory_class="terminator"
     misc_feature    477..529
                     /label=attP Bxb1
                     /note="attP Bxb1"
     misc_RNA        531..581
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     misc_feature    605..689
                     /label=BCD2
                     /note="BCD2"
     RBS             675..683
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             687..1382
                     /label=mKate2
                     /note="monomeric far-red fluorescent protein (Shcherbo et
                     al., 2009)"
     terminator      complement(1408..1451)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1492..1519
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    1526..1546
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      1582..1613
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     terminator      1738..1832
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      2324..2546
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             2594..3541
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     CDS             3833..4690
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      4704..4763
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      4823..4852
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     terminator      complement(4946..4975)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"