Alpha3000-MT-gli2-6xHIS vector (V010120)

Basic Vector Information

      • Vector Name:
      • Alpha3000-MT-gli2-6xHIS
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 11145 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Cerrizuela S, Vega-Lopez GA, Palacio MB, Aybar MJ.

Alpha3000-MT-gli2-6xHIS vector Vector Map

Alpha3000-MT-gli2-6xHIS11145 bp500100015002000250030003500400045005000550060006500700075008000850090009500100001050011000Gli2T7 promoterT7 promoterM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoterM13 fwd

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

Alpha3000-MT-gli2-6xHIS vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported               11145 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector Alpha3000-MT-gli2-6xHIS, complete sequence.
ACCESSION   MF435043
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11145)
  AUTHORS   Cerrizuela S, Vega-Lopez GA, Palacio MB, Aybar MJ.
  TITLE     Gli2 is required for the induction and migration of Xenopus laevis 
            neural crest
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11145)
  AUTHORS   Cerrizuela S, Vega-Lopez GA, Palacio MB, Aybar MJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2017) Developmental Biology, Instituto Superior de
            Investigaciones Biologicas (INSIBIO, CONICET), Chacabuco 461, San 
            Miguel de Tucuman, Tucuman T4000ILI, Argentina
REFERENCE   3  (bases 1 to 11145)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11145
                     /organism="Cloning vector Alpha3000-MT-gli2-6xHIS"
                     /mol_type="other DNA"
                     /db_xref="taxon:2052062"
     CDS             21..4397
                     /codon_start=1
                     /gene="Gli2"
                     /product="Gli2"
                     /label=Gli2
                     /note="full length coding sequence of Xenopus laevis Gli2 
                     protein"
                     /protein_id="ATQ62671.1"
                     /translation="MEQKLISEEDLNEMEQKLISEEDLNEMEQKLISEEDLNEMEQKLI
                     SEEDLNEMEQKLISEEDLNEMESLGDLTMEQKLISEEDLNSRPLDVEHYLRSVHNSPTL
                     SMISAARGLSPAEVAHEHLKERGIYGLAPPPPGTTPTEYCHQMAFLASHASPYGELLVQ
                     SAAAGNTSHLHDYLTPMDVSRFSSPRVTPRLSRKRALSISPLSDASIDLQTMIRTSPNS
                     LVAYINNSRSSSAASGSYGHLSAGAISPAFSFPIHKPCSLSAALSQQRSLSSSFGHTPL
                     LHPSPTFASRQQGALTSANPAPPSNNSSAPDSVLNKVSSESAVSSTVNQVIHKRSKVKT
                     EEEADSVRFPQPPDHLTDLKEDLDKDECKQEPEHIYETNCHWDGCSKEFDTQDQLVHHI
                     NNDHIHGEKKEFVCRWQDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCFKAYSRL
                     ENLKTHLRSHTGEKPYVCDHEGCNKAFSNASDRAKHQNRTHSNEKPYICKVPGCTKRYT
                     DPSSLRKHVKTVHGPEAHVTKKHRNDIIQKPSLPKENGDNEASAKLSGREHSDSVSRDQ
                     EHCLQTRTIKTEDNMMHQSSPGGQSSCSSEPSPYGNTNNIDSGVDVSLAWQGSLGDLFG
                     LEETSPVVDSTVSSWQRSGRPATPETQRIHSAETGTAEEREIKDNERFLLIYEPNATCQ
                     NTRLPTISANGFDVIGVPSSVLINPRAIELSMNDVTMMNQLNERRDSTSSTLSSAYTSR
                     RSSGISPYFSSRRSSETSQFGGRLNNSSSADSYDPISTDASRRSSEASQHSGLPNLLNL
                     TPAQHYRLKAKYAAATGGPPPTPLPNMDRIGLRNKLSLMDGADFPLPPFRQLPVPRRCS
                     DGGGNAGLTPMYPHEIPGNNSRRASDPVRRTAGIDDKPLPRFSRFHSMNSMNTLHPPSL
                     SERRNGGLQHYTCSDGGLHRHVYSPRPPSISENVAMEAISCDADVPGGDDDLMLPDDVV
                     QYIRSQNREAPEQNLQTEYSSPARNLQSNTKSFHNNTPEQPRAPGAYLSRNFPALAECL
                     GQTANMQDNNMPVQWNEVSSGTVDVSDLPKQQFAAGNLAVVQQKQNFAQYQSFNQAPMQ
                     RAHNIMGQGQESVQRNISVNGQRFNYLQQRQQQMSQCQIVSSDFIPQQRYSQSQSMLSS
                     RAMQEGQSQISPSCNNMVERPGVHTHAAPSNTLNHQRLAVHGAPTQGFANNFSVNQDGL
                     HPPNAYTVQPQKNGLEPQQNTLGMSGQAFNHGMIQPRPPAAPHPPNRPRNIHPVHHPPY
                     MRSPHPVSELSPGQQTAEATPKRTSENTDPTPKDNNLLYYSGQIHMYEPNGNFGSGIDC
                     TVRQLPTMPSPGANQVTSTVDSQGLEHPPVIDFDAMMDDGDHSSLMSGTLSPGLLQSFS
                     QTSSRLTTPRNSLTLPSIPAGINNMAIGDMSSMLTTLAEESKFLAIMQLQHHHHHH"
     gene            21..4397
                     /gene="Gli2"
                     /label=Gli2
     sig_peptide     21..275
                     /gene="Gli2"
     CDS             24..53
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             63..92
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             102..131
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             141..170
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             180..209
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             243..272
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     sig_peptide     4377..4394
                     /gene="Gli2"
     CDS             4377..4394
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(4405..4422)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4427..4622
                     /regulatory_class="polyA_signal_sequence"
     polyA_signal    4427..4561
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(4660..4678)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5108..5124)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5132..5148
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5156..5186)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5201..5222
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5510..6098)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6269..7129)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7130..7234)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7708..7724
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      8046..11130
                     /regulatory_class="promoter"
     regulatory      10953..10958
                     /regulatory_class="TATA_box"

This page is informational only.