Alpha-3000-gli3Act vector (V010121)

Basic Vector Information

      • Vector Name:
      • Alpha-3000-gli3Act
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 10139 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Vega Lopez GA, Cerrizuela S, Palacio MB, Aguero TH, Tribulo C, Aybar MJ.

Alpha-3000-gli3Act vector Vector Map

Alpha-3000-gli3Act10139 bp50010001500200025003000350040004500500055006000650070007500800085009000950010000

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

Alpha-3000-gli3Act vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported               10139 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector Alpha-3000-gli3Act, complete sequence.
ACCESSION   KY435756
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10139)
  AUTHORS   Vega Lopez GA, Cerrizuela S, Palacio MB, Aguero TH, Tribulo C, Aybar
            MJ.
  TITLE     The gli3 transcriptional activator is a key effector of Indian 
            hedgehog signaling during Xenopus neural crest development
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10139)
  AUTHORS   Vega Lopez GA, Cerrizuela S, Palacio MB, Aguero TH, Tribulo C, Aybar
            MJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2017) Developmental Biology, Instituto Superior de
            Investigaciones Biologicas (INSIBIO, CONICET-UNT), Chacabuco 461, 
            Tucuman, Tucuman T4000ILI, Argentina
REFERENCE   3  (bases 1 to 10139)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: vector nti v. 11
            Sequencing Technology :: Applied Biosystems 3130 Genetic Analyzer 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10139
                     /organism="Cloning vector Alpha-3000-gli3Act"
                     /mol_type="other DNA"
                     /label=sleeping beauty vector
                     /note="sleeping beauty vector"
                     /db_xref="taxon:1969589"
     regulatory      64..7119
                     /regulatory_class="promoter"
                     /gene="Xlp-Sla-3000"
     gene            64..7119
                     /gene="Xlp-Sla-3000"
                     /label=Xlp-Sla-3000
     CDS             85..3606
                     /codon_start=1
                     /gene="Xlp-Sla-3000"
                     /product="gli3-Act"
                     /label=Xlp-Sla-3000
                     /note="Activator form of GLI3 transcription factor"
                     /protein_id="ARB02201.1"
                     /translation="MPGIPSVLNPVQVSIGPSEAAQQNKPTSESAVSSTGDLLHNKRSK
                     VKPEEDHPSPGAVCIQDQPDGMTLVKEEGVKDESKQEAEVVYETNCHWEGCSREFDTQE
                     QLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCS
                     KAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPG
                     CTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPREPGSHSQSRSPGQQTQGI
                     HGEHKDLSNTTSKHEECLQVRSVKTEKPMSSQPSPGGKSSCSRQQSPISNYTNSGIELN
                     LNSGGSIGDFSALDETPIMDSTISTATTGLGLHGRRVMTGTKWMEQIKVEKMKQVNGML
                     PRLNPVPPHRAPTLPPITGNGVQLNSNISLGGLAPVLPNRNDLSSMDITVLNMLNRRDS
                     NTSTISSAYMSSRRSSGISPCFSSRRSSEASQFEGRVQNLSVAGSYDPISTDASRRSSE
                     TSQCDGLPTLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRMALLGDSRDCR
                     LSPLPSVNVPRRCSDGGVGSYGRRHLLPNELQVNGMRRASDPVKMVSDNVSNTRVVRFN
                     SLNNVIPPDVPPPMERRNLSLQNYTRNVYSPCPPSISENVALEAMIMEAEGNFDDEDLL
                     PDDMVQYLNSRNQRLYETIETDALQNNAGERNGNNFEQSHVTSNVINEQFHSSEQTDLI
                     ENKNDLPIQWNEVSSGSAEYSPSRLKYGQRFPTQQNQPFGLYNNMMVQQQNVPKSGLSQ
                     QRGYQHHTQNNPQAPQQNLDLYNNSNVWSGQLGRGNQYIDDIERSSLGHSATGSLCNSA
                     ARGQKLTTNGLPMNTGQQNFGPSTHYSVQSANRAEVVQNENIINQEFMQQMTAEHLIYG
                     MHLLGVTRSNQTTSGQNGNTTDGTRSFLSTTQNGGEQQPTLAKNFQSFVNSSGNSRQNL
                     HRNNLPYLQEQIYDTNQHIFKVNSIKMEMQSHPQQHCANAQNFSGQLYDQTATYPQQIM
                     KSGSEPGMEANCLLQESNNTNSSKLLSPGANHVTSTVDNIDNSLAGVQIAFAAIIDDGD
                     HASLISGVLSPSIIQNLSRNSSRLTTPRASLTFPAIPVSTSNMAIGDMSSLLTSLAEES
                     KFLAIMQ"
     promoter        complement(3614..3631)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3636..3770
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4181..4197)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4205..4221
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4229..4259)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4274..4295
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4583..5171)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5342..6202)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="Ampicilin resistance"
                     /protein_id="ARB02200.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6203..6307)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6781..6797
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"

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