Basic Vector Information
- Vector Name:
- Alpha-3000-gli2
- Antibiotic Resistance:
- Ampicillin
- Length:
- 11124 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Cerrizuela S, Vega-Lopez GA, Palacio MB, Aybar MJ.
Alpha-3000-gli2 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
Alpha-3000-gli2 vector Sequence
LOCUS 40924_225 11124 bp DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector Alpha-3000-gli2, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11124) AUTHORS Cerrizuela S, Vega-Lopez GA, Palacio MB, Aybar MJ. TITLE Gli2 is required for the induction and migration of Xenopus laevis neural crest JOURNAL Unpublished REFERENCE 2 (bases 1 to 11124) AUTHORS Cerrizuela S, Vega-Lopez GA, Palacio MB, Aybar MJ. TITLE Direct Submission JOURNAL Submitted (05-JUL-2017) Developmental Biology, Instituto Superior de Investigaciones Biologicas (INSIBIO, CONICET), Chacabuco 461, San Miguel de Tucuman, Tucuman T4000ILI, Argentina REFERENCE 3 (bases 1 to 11124) TITLE Direct Submission REFERENCE 4 (bases 1 to 11124) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (05-JUL-2017) Developmental Biology, Instituto Superior de Investigaciones Biologicas (INSIBIO, CONICET), Chacabuco 461, San Miguel de Tucuman, Tucuman T4000ILI, Argentina" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..11124 /mol_type="other DNA" /organism="synthetic DNA construct" gene 21..275 /gene="Myc Tag" /label=Myc Tag sig_peptide 21..275 /gene="Myc Tag" CDS 24..53 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 63..92 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 102..131 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 141..170 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 180..209 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 243..272 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 417..4370 /gene="gli4" /label=gli4 /note="Zinc finger protein GLI4 from Xenopus laevis. Accession#: Q91661" promoter complement(4384..4401) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" polyA_signal complement(4406..4540) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" promoter complement(4639..4657) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(5087..5103) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 5111..5127 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5135..5165) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(5180..5201) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(5489..6077) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(6251..7108) /label=AmpR /note="beta-lactamase" promoter complement(7109..7213) /label=AmpR promoter primer_bind 7687..7703 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" regulatory 8025..11109 /gene="Xlp-Sla-3000" /regulatory_class="promoter" gene 8025..11109 /gene="Xlp-Sla-3000" /label=Xlp-Sla-3000 regulatory 10932..10937 /gene="Xlp-Sla-3000" /regulatory_class="TATA_box"
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