Basic Vector Information
AF286077 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
AF286077 vector Sequence
LOCUS 40924_205 8799 bp DNA circular SYN 17-DEC-2018
DEFINITION Expression vector AF286077, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8799)
AUTHORS Wang RF, Mullins JI.
TITLE Mammalian cell/vaccinia virus expression vectors with increased
stability of retroviral sequences in Escherichia coli: production of
feline immunodeficiency virus envelope protein
JOURNAL Gene 153 (2), 197-202 (1995)
PUBMED 7875588
REFERENCE 2 (bases 1 to 8799)
AUTHORS Mullins JI, Hanley T, Kafsack BFC., Rowen L.
TITLE Direct Submission
JOURNAL Submitted (11-JUL-2000) Microbiology, University of Washington, HSB
K-455, Seattle, Washington 98195, USA
REFERENCE 3 (bases 1 to 8799)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 8799)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Gene";
date: "1995"; volume: "153"; issue: "2"; pages: "197-202"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(11-JUL-2000) Microbiology, University of Washington, HSB K-455,
Seattle, Washington 98195, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..8799
/mol_type="other DNA"
/organism="synthetic DNA construct"
gene 46..500
/pseudo
/gene="thymidine kinase"
/label=thymidine kinase
/note="derived from vaccinia; 5' end"
promoter 526..1140
/label=SR-alpha promoter
/note="hybrid promoter consisting of the SV40 early
promoter plus part of the long terminal repeat of human
T-lymphotrophic virus 1"
intron 1181..1274
/label=SV40 intron
/note="modified SV40 intron with splice donor and acceptor
sites"
promoter 1302..1320
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 1413..1869
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 1917..1934
/label=6xHis
/note="6xHis affinity tag"
misc_feature 1960..2007
/label=similar to tpa 3' end
/note="similar to tpa 3' end"
polyA_signal 2031..2255
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
regulatory 2301..2331
/label=synthetic
/note="synthetic"
/regulatory_class="polyA_signal_sequence"
terminator 2396..2443
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
regulatory 2474..2747
/label=derived from P7.5 vaccinia
/note="derived from P7.5 vaccinia"
/regulatory_class="promoter"
misc_feature 2777..3143
/label=murine beta-globin transcriptional regulation unit
/note="murine beta-globin transcriptional regulation unit"
CDS 3244..3804
/label=DHFR
/note="mouse dihydrofolate reductase"
polyA_signal 3900..4107
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
misc_feature 4142..4507
/label=murine beta-globin transcriptional regulation unit
/note="murine beta-globin transcriptional regulation unit"
CDS 4528..5319
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
polyA_signal 5496..5630
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
misc_feature 5638..6265
/label=vaccinia thymidine kinase gene cluster 3' end
/note="vaccinia thymidine kinase gene cluster 3' end"
gene 5638..5911
/pseudo
/gene="thymidine kinase"
/label=thymidine kinase
/note="derived from vaccinia; 3' end"
misc_feature 6320..6449
/label=similar to pUC19
/note="similar to pUC19"
rep_origin 6489..7033
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin."
CDS complement(7195..8052)
/label=AmpR
/note="beta-lactamase"
promoter complement(8053..8157)
/label=AmpR promoter
rep_origin complement(8183..8638)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
This page is informational only.