AF286077 vector (V010125)

LOCUS       40924_205        8799 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector AF286077, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8799)
  AUTHORS   Wang RF, Mullins JI.
  TITLE     Mammalian cell/vaccinia virus expression vectors with increased 
            stability of retroviral sequences in Escherichia coli: production of
            feline immunodeficiency virus envelope protein
  JOURNAL   Gene 153 (2), 197-202 (1995)
  PUBMED    7875588
REFERENCE   2  (bases 1 to 8799)
  AUTHORS   Mullins JI, Hanley T, Kafsack BFC., Rowen L.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUL-2000) Microbiology, University of Washington, HSB 
            K-455, Seattle, Washington 98195, USA
REFERENCE   3  (bases 1 to 8799)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8799)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1995"; volume: "153"; issue: "2"; pages: "197-202"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-JUL-2000) Microbiology, University of Washington, HSB K-455, 
            Seattle, Washington 98195, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            46..500
                     /pseudo
                     /gene="thymidine kinase"
                     /label=thymidine kinase
                     /note="derived from vaccinia; 5' end"
     promoter        526..1140
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     intron          1181..1274
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     promoter        1302..1320
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1413..1869
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             1917..1934
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_feature    1960..2007
                     /label=similar to tpa 3' end
                     /note="similar to tpa 3' end"
     polyA_signal    2031..2255
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      2301..2331
                     /label=synthetic
                     /note="synthetic"
                     /regulatory_class="polyA_signal_sequence"
     terminator      2396..2443
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     regulatory      2474..2747
                     /label=derived from P7.5 vaccinia
                     /note="derived from P7.5 vaccinia"
                     /regulatory_class="promoter"
     misc_feature    2777..3143
                     /label=murine beta-globin transcriptional regulation unit
                     /note="murine beta-globin transcriptional regulation unit"
     CDS             3244..3804
                     /label=DHFR
                     /note="mouse dihydrofolate reductase"
     polyA_signal    3900..4107
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    4142..4507
                     /label=murine beta-globin transcriptional regulation unit
                     /note="murine beta-globin transcriptional regulation unit"
     CDS             4528..5319
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5496..5630
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    5638..6265
                     /label=vaccinia thymidine kinase gene cluster 3' end
                     /note="vaccinia thymidine kinase gene cluster 3' end"
     gene            5638..5911
                     /pseudo
                     /gene="thymidine kinase"
                     /label=thymidine kinase
                     /note="derived from vaccinia; 3' end"
     misc_feature    6320..6449
                     /label=similar to pUC19
                     /note="similar to pUC19"
     rep_origin      6489..7033
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(7195..8052)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8053..8157)
                     /label=AmpR promoter
     rep_origin      complement(8183..8638)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"