AF286077 vector (V010125)

Basic Vector Information

      • Vector Name:
      • AF286077
      • Antibiotic Resistance:
      • Kanamycin
      • Length:
      • 8799 bp
      • Type:
      • Expression vector
      • Source/Author:
      • Wang RF, Mullins JI.

AF286077 vector Vector Map

AF2860778799 bp4008001200160020002400280032003600400044004800520056006000640068007200760080008400vaccinia thymidine kinase gene cluster 5' endderived from SV40SR-alpha Hybrid Promoter; formed by HTLV LTR and SV40 promoterderived from EMVpolyhistidine tractsimilar to tpa 3' endderived from bovine growth hormonesyntheticderived from T7derived from P7.5 vacciniamurine beta-globin transcriptional regulation unitDHFRderived from bovine growth hormonemurine beta-globin transcriptional regulation unitneomycin resistancederived from SV40 hormonevaccinia thymidine kinase gene cluster 3' endsimilar to pUC19p15A intermediate copy numbersimilar to pUC19

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

AF286077 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                8799 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector AF286077, complete sequence.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8799)
  AUTHORS   Wang RF, Mullins JI.
  TITLE     Mammalian cell/vaccinia virus expression vectors with increased 
            stability of retroviral sequences in Escherichia coli: production of
            feline immunodeficiency virus envelope protein
  JOURNAL   Gene 153 (2), 197-202 (1995)
  PUBMED    7875588
REFERENCE   2  (bases 1 to 8799)
  AUTHORS   Mullins JI, Hanley T, Kafsack BFC., Rowen L.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUL-2000) Microbiology, University of Washington, HSB 
            K-455, Seattle, Washington 98195, USA
REFERENCE   3  (bases 1 to 8799)
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8799
                     /organism="Expression vector AF286077"
                     /mol_type="other DNA"
     misc_feature    join(1..500,8708..8799)
                     /label=vaccinia thymidine kinase gene cluster 5' end
                     /note="vaccinia thymidine kinase gene cluster 5' end"
     gene            46..500
                     /gene="thymidine kinase"
                     /label=thymidine kinase
                     /note="derived from vaccinia; 5' end"
     regulatory      502..864
                     /note="derived from SV40"
     promoter        526..1140
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      706..841
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      872..1306
                     /note="SR-alpha Hybrid Promoter; formed by HTLV LTR and 
                     SV40 promoter"
     LTR             872..1140
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          1181..1274
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
     promoter        1302..1320
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1344..1892
                     /note="derived from EMV"
     misc_feature    1849..1857
                     /label=oligopyrimidine tract
                     /note="oligopyrimidine tract"
     misc_feature    1917..1934
                     /label=polyhistidine tract
                     /note="polyhistidine tract"
     CDS             1917..1934
                     /product="6xHis affinity tag"
     misc_feature    1960..2007
                     /label=similar to tpa 3' end
                     /note="similar to tpa 3' end"
     regulatory      2018..2288
                     /note="derived from bovine growth hormone"
     polyA_signal    2031..2255
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      2301..2331
     regulatory      2339..2465
                     /note="derived from T7"
     terminator      2396..2443
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
     regulatory      2474..2747
                     /note="derived from P7.5 vaccinia"
     misc_feature    2777..3143
                     /label=murine beta-globin transcriptional regulation unit
                     /note="murine beta-globin transcriptional regulation unit"
     misc_feature    3189..3851
     CDS             3244..3807
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
     regulatory      3888..4141
                     /note="derived from bovine growth hormone"
     polyA_signal    3900..4124
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    4142..4507
                     /label=murine beta-globin transcriptional regulation unit
                     /note="murine beta-globin transcriptional regulation unit"
     gene            4527..5492
                     /gene="neomycin resistance"
                     /label=neomycin resistance
     CDS             4528..5322
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="confers resistance to neomycin, kanamycin, and G418 
     regulatory      5496..5632
                     /note="derived from SV40 hormone"
     polyA_signal    5496..5630
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    5638..6265
                     /label=vaccinia thymidine kinase gene cluster 3' end
                     /note="vaccinia thymidine kinase gene cluster 3' end"
     gene            5638..5911
                     /gene="thymidine kinase"
                     /label=thymidine kinase
                     /note="derived from vaccinia; 3' end"
     misc_feature    6320..6449
                     /label=similar to pUC19
                     /note="similar to pUC19"
     rep_origin      6453..7047
                     /label=p15A intermediate copy number
                     /note="p15A intermediate copy number"
     rep_origin      6489..7033
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     misc_feature    7050..8192
                     /label=similar to pUC19
                     /note="similar to pUC19"
     CDS             complement(7192..8052)
                     /product="beta lactamase"
                     /label=beta lactamase
     promoter        complement(8053..8157)
                     /label=AmpR promoter
     rep_origin      complement(8183..8638)
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     misc_feature    8210..8636
                     /label=f1 packaging origin
                     /note="f1 packaging origin"

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