AbVec-hIgKappa vector (V010128)

LOCUS       40924_195        5074 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector AbVec-hIgKappa, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Di Niro R, Mesin L, Raki M, Zheng NY, Lund-Johansen F, Lundin KE, 
            Charpilienne A, Poncet D, Wilson PC, Sollid LM.
  TITLE     Rapid Generation of Rotavirus-Specific Human Monoclonal Antibodies 
            from Small-Intestinal Mucosa
  JOURNAL   J. Immunol. 185 (9), 5377-5383 (2010)
  PUBMED    20935207
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   Wilson PC.
  TITLE     Ig-kappa expression vector for expression-cloning of antibody 
            variable region genes
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5074)
  AUTHORS   Wilson PC, Wilson PC, Wilson PC.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-NOV-2008) Medicine/Rheumatology, The University of 
            Chicago, BSLC/JFK Bldg. 924 East 57th St., Rm 414, Chicago, IL 
            60637, USA
REFERENCE   4  (bases 1 to 5074)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5074)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Immunol."; date: "2010"; volume: "185"; issue: "9"; pages: 
            "5377-5383"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (18-NOV-2008) Medicine/Rheumatology, The University of Chicago, 
            BSLC/JFK Bldg. 924 East 57th St., Rm 414, Chicago, IL 60637, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             929..985
                     /label=IgH signal sequence
                     /note="signal sequence from the mouse immunoglobulin heavy
                     chain variable region"
     CDS             991..1308
                     /label=hIg-kappa-CL
                     /note="Human immunoglobulin kappa light chain constant
                     region"
     regulatory      1319..1328
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1332..1466
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1539..1896
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(1916..1932)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2145..2600
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2932..3036
                     /label=AmpR promoter
     CDS             3037..3894
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4068..4656
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4944..4965
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4980..5010
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5018..5034
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5042..5058
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"