35SCoreSINCTIF1-pFLEV vector (V010146)

Basic Vector Information

      • Vector Name:
      • 35SCoreSINCTIF1-pFLEV
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 4205 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Grant TN, De La Torre CM, Zhang N, Finer JJ.

35SCoreSINCTIF1-pFLEV vector Vector Map

35SCoreSINCTIF1-pFLEV4205 bp600120018002400300036004200M13 fwdminimal CaMV 35S promotersGTF65TNOS terminatorM13 revlac operatorlac promoterCAP binding siteampRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

35SCoreSINCTIF1-pFLEV vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                4205 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector 35SCoreSINCTIF1-pFLEV, complete sequence.
ACCESSION   KX814444
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Grant TN, De La Torre CM, Zhang N, Finer JJ.
  TITLE     Synthetic introns help identify sequences in the 5' UTR intron of 
            the Glycine max polyubiquitin (Gmubi) promoter that give increased 
            promoter activity
  JOURNAL   Planta (2017) In press
  PUBMED    28070655
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Finer J, Grant T, De La Torre C, Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 4205)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Cloning vector 35SCoreSINCTIF1-pFLEV"
                     /mol_type="other DNA"
                     /db_xref="taxon:1955482"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        447..493
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     regulatory      448..922
                     /regulatory_class="promoter"
                     /note="35SCoreTIF1 synthetic promoter"
     CDS             925..1644
                     /codon_start=1
                     /product="sGTF65T"
                     /label=sGTF65T
                     /protein_id="AQM73596.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     regulatory      1677..1952
                     /regulatory_class="terminator"
                     /note="NOS terminator"
     terminator      1677..1929
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1984..2000)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2008..2024
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2032..2062)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2077..2098
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(2386..2974)
                     /direction=LEFT
     gene            complement(3145..4005)
                     /gene="ampR"
                     /label=ampR
     CDS             complement(3145..4005)
                     /codon_start=1
                     /gene="ampR"
                     /product="ampicillin resistance protein"
                     /label=ampR
                     /protein_id="AQM73595.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4006..4110)
                     /gene="bla"
                     /label=AmpR promoter

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