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pMIGR1-FLAG-BCR-ABL-P210-T1216I vector (V013686)

Basic Vector Information

Vector Name:
pMIGR1-FLAG-BCR-ABL-P210-T1216I
Antibiotic Resistance:
Ampicillin
Length:
12564 bp
Type:
Protein expression
Replication origin:
ori
Host:
Mammalian cells, Retrovirus
Promoter:
MSCV
Growth Strain(s):
Stbl3
Growth Temperature:
37℃

pMIGR1-FLAG-BCR-ABL-P210-T1216I vector Vector Map

pMIGR1-FLAG-BCR-ABL-P210-T1216I12564 bp60012001800240030003600420048005400600066007200780084009000960010200108001140012000FLAGABL1IRES2EGFP3' LTRlac promoterCAP binding siteoriAmpRAmpR promoter5' LTRMESV Psigag (truncated)

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMIGR1-FLAG-BCR-ABL-P210-T1216I vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_16790       12564 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12564)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             156..179
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             2961..6272
                     /gene="ABL1"
                     /label=ABL1
                     /note="Tyrosine-protein kinase ABL1 from Homo sapiens. 
                     Accession#: P00519"
     misc_feature    6284..6860
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             6861..7577
                     /label=EGFP
                     /note="enhanced GFP"
     LTR             7682..8196
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem
                     cell virus"
     promoter        complement(8382..8412)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8427..8448)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8736..9324)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9498..10355)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10356..10460)
                     /label=AmpR promoter
     LTR             11288..11804
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem
                     cell virus"
     misc_feature    11868..12209
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell
                     virus"
     CDS             join(12276..12564,1..128)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"

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