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pPICZαA-mCherry vector (V013657)

Basic Vector Information

Vector Name:
pPICZαA-mCherry
Antibiotic Resistance:
Bleomycin
Length:
4267 bp
Type:
Protein expression
Replication origin:
ori
Host:
Yeast
Selection Marker:
Zeo
Promoter:
AOX1
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pPICZαA-mCherry vector Vector Map

pPICZαA-mCherry4267 bp600120018002400300036004200AOX1 promotermCherry6xHisAOX1 terminatorTEF1 promoterEM7 promoterBleoRCYC1 terminatorori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pPICZαA-mCherry vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_18485        4267 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..940
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     CDS             1277..1984
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             1994..2011
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      2091..2337
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        2352..2763
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        2771..2818
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2837..3208
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      3277..3524
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(3599..4187)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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