Basic Vector Information
- Vector Name:
- pMIGR1-FLAG-BCR-ABL-P210
- Antibiotic Resistance:
- Ampicillin
- Length:
- 12564 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells, Retrovirus
- Promoter:
- MSCV
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
pMIGR1-FLAG-BCR-ABL-P210 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMIGR1-FLAG-BCR-ABL-P210 vector Sequence
LOCUS 62056_16795 12564 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12564) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..12564 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 156..179 /label=FLAG /note="FLAG(R) epitope tag, followed by an enterokinase cleavage site" CDS 2961..6272 /gene="ABL1" /label=ABL1 /note="Tyrosine-protein kinase ABL1 from Homo sapiens. Accession#: P00519" misc_feature 6284..6860 /label=IRES2 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" CDS 6861..7577 /label=EGFP /note="enhanced GFP" LTR 7682..8196 /label=3' LTR /note="3' long terminal repeat from murine embryonic stem cell virus" promoter complement(8382..8412) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(8427..8448) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(8736..9324) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(9498..10355) /label=AmpR /note="beta-lactamase" promoter complement(10356..10460) /label=AmpR promoter LTR 11288..11804 /label=5' LTR /note="5' long terminal repeat from murine embryonic stem cell virus" misc_feature 11868..12209 /label=MESV Psi /note="packaging signal of murine embryonic stem cell virus" CDS join(12276..12564,1..128) /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon"
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