Price Information
| Cat No. | Plasmid Name | Availability | Buy one, get one free! (?) |
|---|---|---|---|
| V013488 | UCOE Mu-H | In stock, instant shipping |
Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.
Basic Vector Information
Ubiquitous Chromatin Opening Element (UCOE) technology significantly enhances gene expression in stably transfected mammalian cells by modulating chromatin structure. Derived from small DNA elements near housekeeping genes, UCOEs create a transcriptionally active open chromatin environment around the integrated transgene. This prevents transgene silencing and enables consistent, stable, and high-level gene expression regardless of the transgene's chromosomal integration site.
- Vector Name:
- UCOE Mu-H
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8182 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- Hyg
- Promoter:
- CMV
- Growth Strain(s):
- DH10B
- Growth Temperature:
- 37℃
UCOE Mu-H vector Map
Plasmid Protocol
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5. Store the plasmid at -20 ℃.
6. The concentration of plasmid re-measurement sometimes differs from the nominal value, which may be due to the position of the lyophilized plasmid in the tube, the efficiency of the re-dissolution, the measurement bias, and adsorption on the wall of the tube, therefore, it is recommended to transform and extract the plasmid before using it
General Plasmid Transform Protocol
1. Take one 100μl of the competent cells and thaw it on ice for 10min, add 2μl of plasmid, then ice bath for 30min, then heat-shock it at 42℃ for 60s, do not stir, and then ice bath for 2min.
2. Add 900μl of LB liquid medium without antibiotics, and incubate at 37℃ for 45min (30℃ for 1-1.5 hours) with 180rpm shaking.
3. Centrifuge at 6000rpm for 5min, leave only 100μl of supernatant to resuspend the bacterial precipitate and spread it onto the target plasmid-resistant LB plate.
4. Invert the plate and incubate at 37℃ for 14h, or at 30℃ for 20h.
5. Pick a single colony into LB liquid medium, add the corresponding antibiotics, incubate at 220rpm for 14h, and extract the plasmid according to the experimental needs and the instructions of the plasmid extraction kit.
References
- González-Pereira P, Trinh R, Vasuthasawat A, Bartsch-Jiménez A, Nuñez-Soto C, Altamirano C. Enhancing Antibody-Specific Productivity: Unraveling the Impact of XBP1s Overexpression and Glutamine Availability in SP2/0 Cells. Bioengineering (Basel). 2024 Feb 21;11(3):201. doi: 10.3390/bioengineering11030201. PMID: 38534475; PMCID: PMC10968409.
UCOE Mu-H vector Sequence
LOCUS Exported 8182 bp DNA circular SYN 03-DEC-2025
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8182)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 8182)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..8182
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 31..47
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 57..75
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 158..174
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 249..3296
/label=UCOE
/note="The Rps3 chromatin opening element (UCOE) is a
fragment of DNA from the mouse Rps3 gene that is used in
biotechnology and gene therapy to maintain stable and
high-level gene expression. It functions as a ubiquitous
chromatin opening element that prevents epigenetic
silencing by keeping the DNA in an open, transcriptionally
active state. "
enhancer 3383..3686
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 3687..3890
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
polyA_signal 4142..4263
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 4387..4890
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
CDS 4925..5947
/codon_start=1
/label=HygR
/note="aminoglycoside phosphotransferase from E. coli"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
promoter complement(6017..6035)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(6056..6072)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(6080..6096)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6104..6134)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(6149..6170)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(6458..7046)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7220..8077)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(8078..8182)
/label=AmpR promoter