Basic Vector Information
- Vector Name:
- pMIGR1-IRES2-VN173
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6476 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells, Retrovirus
- Promoter:
- MSCV
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
pMIGR1-IRES2-VN173 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMIGR1-IRES2-VN173 vector Sequence
LOCUS 62056_16800 6476 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6476) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..6476 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 1..515 /label=MSCV /note="Murine embryonic stem cell virus promoter including the enhancer and promoter region of PCMV virus and 5' untranslated region of dl-587rev retrovirus" misc_feature 579..920 /label=MESV Psi /note="packaging signal of murine embryonic stem cell virus" misc_feature 1437..2022 /label=IRES2 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" CDS 2023..2541 /codon_start=1 /label=VN173 /note="N-terminal fragment of mVenus for use in bimolecular fluorescence complementation (BiFC) (Kodama and Hu, 2010)" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK ANFKIRHNIE" LTR 2838..3352 /label=3' LTR /note="3' long terminal repeat from murine embryonic stem cell virus" primer_bind complement(3521..3537) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(3545..3561) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3569..3599) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(3614..3635) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(3923..4511) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4685..5542) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(5543..5647) /label=AmpR promoter LTR 6475..6476 /label=5' LTR /note="5' long terminal repeat from murine embryonic stem cell virus"
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