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pMUTIN4-GFP vector (V013029)

Basic Vector Information

Vector Name:
pMUTIN4-GFP
Antibiotic Resistance:
Ampicillin
Length:
6416 bp
Type:
Protein expression
Replication origin:
ori
Host:
Bacillus subtilis
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pMUTIN4-GFP vector Vector Map

pMUTIN4-GFP6416 bp300600900120015001800210024002700300033003600390042004500480051005400570060006300lac operatorCycle 3 GFPbomoriAmpRAmpR promoterrRNA adenine N-6-methyltransferaserrnB T1 terminatorrrnB T2 terminatorlambda t0 terminator

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMUTIN4-GFP vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V013029                 6416 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013029
VERSION     V013029
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    334..350
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             420..1136
                     /label="Cycle 3 GFP"
                     /note="Cycle 3 GFP (Crameri et al., 1996)"
     misc_feature    2685..2825
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3011..3599)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3773..4630)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4631..4735)
                     /label="AmpR promoter"
     CDS             5115..5849
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     terminator      6163..6249
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      6268..6295
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      6371..6405
                     /label="lambda t0 terminator"
                     /note="minimal transcription terminator from phage lambda
                     (Scholtissek and Grosse, 1987)"

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