pPA-TagRFP-H2B vector (V012837)

Price Information

Cat No.	Plasmid Name	Availability	Add to cart
V012837	pPA-TagRFP-H2B	In stock (lyophilized plasmid)	Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

pPA-TagRFP-H2B is a mammalian expression vector, which encoding the PA-TagRFP-H2B fusion protein. The vector can be used for fluorescent labeling of histone H2B in living cells.

Vector Name:: pPA-TagRFP-H2B

Antibiotic Resistance:: Kanamycin

Length:: 5089 bp

Type:: Fluorescent Protein Genes & Plasmids

Replication origin:: ori

Source/Author:: Evrogen

Selection Marker:: Neomycin (select with G418)

Copy Number:: High copy number

Promoter:: CMV

Cloning Method:: Enzyme digestion and ligation

Fusion Tag:: PA-TagRFP

Growth Strain(s):: Top10

Growth Temperature:: 37℃

pPA-TagRFP-H2B vector Vector Map

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Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

References

Nijenhuis W, Vallardi G, Teixeira A, Kops GJ, Saurin AT. Negative feedback at kinetochores underlies a responsive spindle checkpoint signal. Nat Cell Biol. 2014 Dec;16(12):1257-64.

pPA-TagRFP-H2B vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_34037        5089 bp DNA     circular SYN 01-MAR-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             657..1034
                     /label=H2B
                     /note="human histone H2B"
     CDS             1053..1751
                     /label=PA-TagRFP
                     /note="photoactivatable variant of the monomeric red
                     fluorescent protein TagRFP (Subach et al., 2010)"
     polyA_signal    1877..1998
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2005..2460)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2487..2591
                     /label=AmpR promoter
     promoter        2593..2950
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2985..3776
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4011..4058
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4387..4975
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"