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pAAV-IRES-tdTomato vector (V012832)

Basic Vector Information

tdTomato is a basic (constitutively fluorescent) orange fluorescent protein published in 2004, derived from Discosoma sp.. It is reported to be a somewhat slowly-maturing tandem dimer with low acid sensitivity.

Vector Name:
pAAV-IRES-tdTomato
Antibiotic Resistance:
Ampicillin
Length:
6636 bp
Type:
Viral Expression & Packaging Vectors
Replication origin:
ori
Selection Marker:
tdTomato
Promoter:
CMV
Growth Strain(s):
Stbl3
Growth Temperature:
37℃
Expression Method:
Constiutive, Stable / Transient

pAAV-IRES-tdTomato vector Map

Copy Sequence View Map
pAAV-IRES-tdTomato6636 bp3006009001200150018002100240027003000330036003900420045004800510054005700600063006600AAV2 ITRCMV enhancerCMV promoterchimeric introntdTomatohGH poly(A) signalAAV2 ITRf1 oriAmpR promoterAmpRori

pAAV-IRES-tdTomato vector Sequence

LOCUS       pAAV-IRES-tdToma        6636 bp DNA     circular SYN 23-SEP-2015
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-IRES-tdTomato
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6636)
  AUTHORS   Youbio
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6636
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     enhancer        221..524
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        525..727
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          876..1254
                     /label=chimeric intron
                     /note="fusion between human cytomegalovirus intron A and
                     human beta-globin intron 2"
     misc_feature    1366..1943
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             1940..3367
                     /label=tdTomato
                     /note="tandem dimeric (pseudo-monomeric) derivative of
                     DsRed (Shaner et al., 2004)"
     polyA_signal    3383..3859
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     repeat_region   3899..4039
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4114..4569
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4851..4955
                     /label=AmpR promoter
     CDS             4956..5813
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5987..6575
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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