pGEM-HE vector (V012814)

Price Information

Cat No. Plasmid Name Availability Add to cart
V012814 pGEM-HE In stock, instant shipping

Buy one, get one free!

Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.

Basic Vector Information

Xenopus oocyte expression vector. It contains a 3'-UTR of the major beta-globin gene of Xenopus laevis. The pGEM-HE is a derivative of pGEM3Z vector.

Vector Name:
pGEM-HE
Antibiotic Resistance:
Ampicillin
Length:
3047 bp
Type:
Protein Expression plasmid
Replication origin:
ori
Selection Marker:
Ampicillin, 100 μg/mL
Copy Number:
High copy number
Promoter:
T7
Growth Strain(s):
DH5alpha
Growth Temperature:
37℃

pGEM-HE vector Vector Map

pGEM-HE3047 bp6001200180024003000M13 fwdT7 promoter5'UTR3'UTRSP6 promoterM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

References

  • Loewen SK, Yao SY, Slugoski MD, Mohabir NN, Turner RJ, Mackey JR, Weiner JH, Gallagher MP, Henderson PJ, Baldwin SA, Cass CE, Young JD. Transport of physiological nucleosides and anti-viral and anti-neoplastic nucleoside drugs by recombinant Escherichia coli nucleoside-H(+) cotransporter (NupC) produced in Xenopus laevis oocytes. Mol Membr Biol. 2004 Jan-Feb;21(1):1-10.

pGEM-HE vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pGEM-HE.        3047 bp DNA     circular SYN 03-AUG-2023
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pGEM-HE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3047)
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..62
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        69..87
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           119..161
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     3'UTR           220..345
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus
                     laevis"
     promoter        complement(457..475)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(493..509)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(517..533)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(541..571)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(586..607)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(895..1483)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1657..2514)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2515..2619)
                     /label=AmpR promoter