Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V012800 | Super PiggyBac Transposase | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
The PiggyBac (PB) transposon is a mobile genetic element that efficiently transposes between vectors andchromosomes via a "cut and paste" mechanism. During transposition, the PB transposase recognizes transposonspecific inverted terminal repeat sequences (ITRs) located on both ends of the transposon vector and efficiently moves the contents from the original sites and efficiently integrates them into TTAA chromosomal sites. The powerful activity of the piggyBac transposon system enables genes of interest between the two ITRs in the PB vector to be easily mobilized into target genomes. The Super PiggyBac transposase expression vector features a 5’ HS4 insulator to support robust transcription from the rPolr2A promoter. The PiggyBac transposase coding sequence has been optimized for high expression, stability and activity in mammalian cells.
The sequencing results indicated the XhoI site was destroyed by the insertion GTCGACGCCGCT.
Super PiggyBac Transposase vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
References
- Sutrave G, Xu N, Tang TCY, Dolnikov A, Gloss B, Gottlieb DJ, Micklethwaite KP, Gowrishankar K. Characterizing piggyBat-a transposase for genetic modification of T cells. Mol Ther Methods Clin Dev. 2022 Mar 22;25:250-263.
Super PiggyBac Transposase vector Sequence
LOCUS Super_PiggyBac_T 8531 bp DNA circular SYN 19-MAY-2023
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS Super PiggyBac Transposase.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8531)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..8531
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 18..1993
/label=chicken 5'-HS4 beta-globin insulator (HS4)
/note="Insulators are DNA sequence elements that prevent
inappropriate interactions between adjacent chromatin
domains. One type of insulator establishes domains that
separate enhancers and promoters to block their
interaction, whereas a second type creates a barrier
against the spread of heterochromatin."
primer_bind 2449..2465
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 2492..3197
/label=Polr2A promoter
/note="RNA polymerase II large subunit promoter"
intron 3442..3538
/label=SV40 intron
/note="modified SV40 intron with splice donor and acceptor
sites"
CDS 3614..5395
/label=hyPBase
/note="Hyperactive version of piggyBac transposase (PBase)
created by mutagenesis"
polyA_signal complement(5424..5558)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter complement(5675..5693)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(5714..5730)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(5738..5754)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5762..5792)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(5807..5828)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(6116..6704)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6878..7735)
/label=AmpR
/note="beta-lactamase"
promoter complement(7736..7840)
/label=AmpR promoter
rep_origin 7867..8322
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 8463..8479
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 8489..8507
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"