pCMV-dR8.2 dvpr vector (V012794)

MSDS

Basic Vector Information

2nd generation lentiviral packaging plasmid. Can be used with 2nd and 3rd generation transfer vectors. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.

Vector Name:: pCMV-dR8.2 dvpr

Antibiotic Resistance:: Ampicillin

Length:: 13380 bp

Type:: Viral Expression & Packaging Vectors

Replication origin:: ori

Source/Author:: Bob Weinberg

Copy Number:: High copy number

Promoter:: CMV

pCMV-dR8.2 dvpr vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pCMV-dR8.2 dvpr vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       addgene-plasmid-       13380 bp DNA     circular SYN 13-DEC-2021
DEFINITION  2nd generation lentiviral packaging plasmid. Can be used with 2nd 
            and 3rd generation transfer vectors. Use in conjunction with an 
            envelope plasmid such as pCMV-VSV-G..
ACCESSION   .
VERSION     .
KEYWORDS    addgene-plasmid-8455-sequence-327823.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13380)
  AUTHORS   Stewart SA, Dykxhoorn DM, Palliser D, Mizuno H, Yu EY, An DS, 
            Sabatini DM, Chen IS, Hahn WC, Sharp PA, Weinberg RA, Novina CD
  TITLE     Lentivirus-delivered stable gene silencing by RNAi in primary cells.
  JOURNAL   RNA 2003 Apr;9(4):493-501.
  PUBMED    12649500
REFERENCE   2  (bases 1 to 13380)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 13380)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA"; date:
            "2003-04"; volume: "9(4)"; pages: "493-501"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             855..2354
                     /label=HIV-1 gag
                     /note="gag protein from human immunodeficiency virus 1"
     CDS             2150..5158
                     /label=HIV-1 pol
                     /note="pol protein from human immunodeficiency virus 1"
     CDS             5106..5681
                     /gene="vif"
                     /label=vif
                     /note="Virion infectivity factor from Human
                     immunodeficiency virus type 1 group M subtype B (isolate 
                     NY5). Accession#: P12504"
     CDS             5821..5994
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate BH5). Accession#: P04612"
     CDS             6052..6294
                     /gene="vpu"
                     /label=vpu
                     /note="Protein Vpu from Human immunodeficiency virus type 1
                     group M subtype B (isolate BH10). Accession#: P69699"
     misc_feature    6458..6691
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6876..6920
                     /label=gp41 peptide
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et 
                     al., 2013)"
     CDS             7069..7110
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     CDS             7214..7240
                     /label=NES
                     /note="nuclear export signal from the HIV Rev protein
                     (Fischer et al., 1995)"
     CDS             7486..8103
                     /gene="nef"
                     /label=nef
                     /note="Protein Nef from Human immunodeficiency virus type 1
                     group M subtype B (isolate LW123). Accession#: Q70627"
     promoter        complement(8477..8495)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8785..8804)
                     /label=pBRrevBam
                     /note="pBRrevBam"
                     /note="pBR322 vectors, tet region, downstream of BamHI,
                     reverse primer"
     promoter        9283..9387
                     /label=AmpR promoter
     CDS             9388..10245
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      10419..11007
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        11253..11582
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     intron          12716..12781
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12911..12931
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"

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