MSCV-IRES-GFP vector (V012785)

Basic Vector Information

      • Vector Name:
      • MSCV-IRES-GFP
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 6488 bp
      • Type:
      • Viral Expression & Packaging Vectors
      • Source/Author:
      • Tannishtha Reya
      • Copy Number:
      • High copy number
      • Promoter:
      • CMV
      • 5' Primer:
      • pLXSN5; pBABE5
      • 3' Primer:
      • pCDH-rev (GCATTCCTTTGGCGAGAG)
      • Fusion Tag:
      • EGFP

MSCV-IRES-GFP vector Vector Map

MSCV-IRES-GFP6488 bp300600900120015001800210024002700300033003600390042004500480051005400570060006300AmpRAmpR promoter5' LTRMESV Psigag (truncated)IRESEGFP3' LTRlac operatorlac promoterCAP binding site

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

MSCV-IRES-GFP vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                6488 bp ds-DNA     circular SYN 16-NOV-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    MSCV-IRES-GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6488)
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6488
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(455..1315)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1316..1420)
                     /gene="bla"
                     /note="AmpR promoter"
     LTR             2248..2764
                     /note="5' LTR"
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    2828..3169
                     /note="MESV Psi"
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             3236..3652
                     /note="gag (truncated)"
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     misc_feature    3686..4246
                     /note="IRES"
     CDS             4249..4968
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="EGFP"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     LTR             5130..5644
                     /note="3' LTR"
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    5806..5822
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5830..5860)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5875..5896
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6184..284)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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