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RNAi-Ready pSIREN-RetroQ-DsRed-Express vector (V012741)

Basic Vector Information

Vector Name:
RNAi-Ready pSIREN-RetroQ-DsRed-Express
Antibiotic Resistance:
Ampicillin
Length:
6543 bp
Type:
RNAi
Replication origin:
ori
Source/Author:
Clontech
Selection Marker:
DsRed
Copy Number:
Low copy number
Promoter:
U6
Expression Method:
Transient

RNAi-Ready pSIREN-RetroQ-DsRed-Express vector Map

Copy Sequence View Map
RNAi-Ready pSIREN-RetroQ-DsRed-Express6543 bp300600900120015001800210024002700300033003600390042004500480051005400570060006300CMV enhancerCMV promoterDsRed-Express3' LTR (Delta-U3)SV40 promoteroriAmpRAmpR promoterCMV enhancerCMV promoter5' LTR (truncated)MMLV Psigag (truncated)U6 promoter

RNAi-Ready pSIREN-RetroQ-DsRed-Express vector Sequence

LOCUS       Untitled_2.        6543 bp DNA     circular SYN 11-SEP-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    Untitled 2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6543)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6543)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        66..369
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        370..573
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             618..1292
                     /label=DsRed-Express
                     /note="rapidly maturing tetrameric variant of DsRed
                     fluorescent protein (Bevis and Glick, 2002)"
     LTR             1415..1840
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     promoter        2106..2435
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(2728..3316)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3490..4347)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4348..4452)
                     /label=AmpR promoter
     enhancer        4638..5017
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5018..5221
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             5222..5397
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    5460..5659
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             5860..6276
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     promoter        6300..6540
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"

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