RNAi-Ready pSIREN-RetroQ-DsRed-Express vector (V012741)

MSDS

Basic Vector Information

Vector Name:: RNAi-Ready pSIREN-RetroQ-DsRed-Express

Antibiotic Resistance:: Ampicillin

Length:: 6543 bp

Type:: RNAi

Replication origin:: ori

Source/Author:: Clontech

Selection Marker:: DsRed

Copy Number:: Low copy number

Promoter:: U6

Expression Method:: Transient

RNAi-Ready pSIREN-RetroQ-DsRed-Express vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

RNAi-Ready pSIREN-RetroQ-DsRed-Express vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Untitled_2.        6543 bp DNA     circular SYN 11-SEP-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    Untitled 2.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6543)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6543)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        66..369
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        370..573
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             618..1292
                     /label=DsRed-Express
                     /note="rapidly maturing tetrameric variant of DsRed
                     fluorescent protein (Bevis and Glick, 2002)"
     LTR             1415..1840
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     promoter        2106..2435
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(2728..3316)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3490..4347)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4348..4452)
                     /label=AmpR promoter
     enhancer        4638..5017
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5018..5221
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             5222..5397
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    5460..5659
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             5860..6276
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     promoter        6300..6540
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"

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