Basic Vector Information
- Vector Name:
- pLVX-EF1α-Luc-N1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10441 bp
- Type:
- Viral Expression & Packaging Vectors
- Replication origin:
- ori
- Source/Author:
- Clontech
- Selection Marker:
- Puromycin
- Copy Number:
- High copy number
- Promoter:
- EF-1α
- Cloning Method:
- Enzyme Cut
- Fusion Tag:
- luciferase
- Expression Method:
- Constiutive, Stable
pLVX-EF1α-Luc-N1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLVX-EF1α-Luc-N1 vector Sequence
LOCUS pLVX-EF1-alpha-L 10441 bp DNA circular SYN 08-APR-2021
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS pLVX-EF1-alpha-Luc-N1.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10441)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 10441)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..10441
/mol_type="other DNA"
/organism="synthetic DNA construct"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1721..1765
/label=gp41 peptide
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 1914..1955
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
misc_feature 2027..2144
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2338..3519
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
misc_feature 3534..3599
/label=MCS
/note="multiple cloning site"
CDS 3606..5255
/label=luciferase
/note="firefly luciferase"
promoter 5276..5775
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
CDS 5796..6392
/label=PuroR
/note="puromycin N-acetyltransferase"
misc_feature 6409..6997
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
LTR 7204..7837
/label=5' LTR
/note="5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(7966..7982)
/label=M13 rev
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 7990..8006
/label=lac repressor encoded by lacI binding site
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8014..8044)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(8059..8080)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(8368..8956)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(9130..9987)
/label=AmpR
/note="beta-lactamase"
promoter complement(9988..10092)
/label=AmpR promoter
polyA_signal 10140..10274
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
This page is informational only.