Price Information
Cat No. | Plasmid Name | Availability | Add to cart |
---|---|---|---|
V012716 | pLVX-PAmCherry-N1 | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
The pLVX-PAmCherry-N1 is a Lentiviral Gene Expression with Photoactivatable Fluorescence. The pLVX-PAmCherry-C1 vector is an HIV-1-based lentiviral expression vector designed to efficiently infect cells and express your gene of interest. The lentiviral particles derived from this vector encode a photoactivatable fluorescent protein, PAmCherry, fused to your gene of interest. PAmCherry, a mutant of mCherry, exhibits no fluorescence until photoactivated by exposure to light within the 350-400 nm wavelength range. Upon activation, PAmCherry emits light at 564 nm (excitation) and 595 nm (emission). Genes cloned into the vector's MCS will be expressed as fusion proteins at the C-terminus of PAmCherry, provided they are in-frame and lack intervening stop codons. This allows for precise expression control and visualization of your protein of interest.
- Vector Name:
- pLVX-PAmCherry-N1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8777 bp
- Type:
- Viral Expression & Packaging Vectors
- Replication origin:
- ori
- Source/Author:
- Clontech
- Selection Marker:
- Puromycin
- Copy Number:
- High copy number
- Promoter:
- mPGK
- Cloning Method:
- Enzyme Cut
- Fusion Tag:
- PAmCherry
- Growth Strain(s):
- stbl3
- Growth Temperature:
- 37℃
- Expression Method:
- Constiutive, Stable
pLVX-PAmCherry-N1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
References
- Wang X, Wu Z, Li Y, Yang Y, Xiao C, Liu X, Xiang X, Wei J, Shao D, Liu K, Deng X, Wu J, Qiu Y, Li B, Ma Z. p53 promotes ZDHHC1-mediated IFITM3 palmitoylation to inhibit Japanese encephalitis virus replication. PLoS Pathog. 2020 Oct 27;16(10):e1009035. doi: 10.1371/journal.ppat.1009035. PMID: 33108395; PMCID: PMC7647115.
pLVX-PAmCherry-N1 vector Sequence
LOCUS pLVX-PAmCherry-N 8777 bp DNA circular SYN 08-APR-2021 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS pLVX-PAmCherry-N1. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8777) TITLE Direct Submission REFERENCE 2 (bases 1 to 8777) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..8777 /mol_type="other DNA" /organism="synthetic DNA construct" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /label=gp41 peptide /note="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" CDS 1914..1955 /note="Protein Tat from Human immunodeficiency virus type 1 group M subtype B (isolate WMJ22). Accession#: P12509" misc_feature 2027..2144 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" enhancer 2201..2504 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2505..2708 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" misc_feature 2802..2867 /label=MCS /note="multiple cloning site" CDS 2874..3581 /label=PAmCherry1 /note="photoactivatable monomeric derivative of DsRed fluorescent protein (Subach et al., 2009)" promoter 3612..4111 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" CDS 4132..4728 /label=PuroR /note="puromycin N-acetyltransferase" misc_feature 4745..5333 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" LTR 5540..6173 /label=5' LTR /note="5' long terminal repeat (LTR) from HIV-1" primer_bind complement(6302..6318) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(6326..6342) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6350..6380) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(6395..6416) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(6704..7292) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7466..8323) /label=AmpR /note="beta-lactamase" promoter complement(8324..8428) /label=AmpR promoter polyA_signal 8476..8610 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal"