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pSilencer adeno 1.0-CMV vector (V012706)

Basic Vector Information

Ambion's pSilencer™ adeno 1.0-CMV System combines the advantages of adenoviral vectors with an efficient siRNA expression system. With pSilencer™ adeno, you can easily deliver an siRNA expressing construct into a variety of mammalian cells and organisms.

Vector Name:
pSilencer adeno 1.0-CMV
Antibiotic Resistance:
Ampicillin
Length:
5891 bp
Type:
RNAi
Replication origin:
ori
Source/Author:
Thermo Fisher Scientific
Copy Number:
High copy number
Promoter:
CMV
Cloning Method:
Enzyme Cut
Expression Method:
Constiutive, Stable

pSilencer adeno 1.0-CMV vector Map

Copy Sequence View Map
pSilencer adeno 1.0-CMV5891 bp60012001800240030003600420048005400ITRAd5 PsiCMV enhancerCMV promoterHexon-interlacing proteinPackaging protein 1oriAmpRAmpR promoter

pSilencer adeno 1.0-CMV vector Sequence

LOCUS       V012706                 5891 bp    DNA     circular SYN 08-APR-2021
DEFINITION  Exported.
ACCESSION   V012706
VERSION     V012706
KEYWORDS    pSilencer adeno 1.0-CMV
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5891)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   16..118
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     misc_signal     206..356
                     /label="Ad5 Psi"
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        447..750
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        751..954
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             1397..1816
                     /gene="IX"
                     /label="Hexon-interlacing protein"
                     /note="Hexon-interlacing protein from Human adenovirus C
                     serotype 5. Accession#: P03281"
     CDS             complement(1882..3216)
                     /gene="IVa2"
                     /label="Packaging protein 1"
                     /note="Packaging protein 1 from Human adenovirus C serotype
                     5. Accession#: P03271"
     rep_origin      complement(4069..4657)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4831..5688)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5689..5793)
                     /label="AmpR promoter"

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