Basic Vector Information
Mammalian expression of Cre recombinase from the broadly active CAG promoter/enhancer
- Vector Name:
- pCAG-Cre
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5871 bp
- Type:
- CRE/LOXP
- Replication origin:
- ori
- Source/Author:
- Dr. Connie Cepko's lab
- Copy Number:
- High copy number
- Promoter:
- chicken β-actin
- Expression Method:
- Transient
pCAG-Cre vector Map
pCAG-Cre vector Sequence
LOCUS pCAG-Cre. 5871 bp DNA circular SYN 06-APR-2021
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS pCAG-Cre
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5871)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 5871)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5871
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 4..383
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 386..661
/note="chicken beta-actin promoter"
intron 662..1670
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
CDS 1737..2765
/label=Cre
/note="site-specific recombinase"
CDS 2775..2804
/label=Myc
/note="Myc (human c-Myc proto-oncogene) epitope tag"
polyA_signal 2960..3015
/label=beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(3374..3390)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(3398..3414)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3422..3452)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(3467..3488)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 3546..3742
/label=SV40 promoter
/note="SV40 early promoter"
polyA_signal 3748..3882
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(4121..4709)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4883..5740)
/label=AmpR
/note="beta-lactamase"
promoter complement(5741..5845)
/label=AmpR promoter
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