Basic Vector Information
- Vector Name:
- Stat3 Flag pRc/CMV
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7969 bp
- Type:
- Mammalian Expression
- Replication origin:
- ori
- Selection Marker:
- Neomycin (select with G418)
- Copy Number:
- High Copy
- Promoter:
- CMV
- Cloning Method:
- Restriction Enzyme
- 5' Primer:
- T7
- 3' Primer:
- SP6
Stat3 Flag pRc/CMV vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
Stat3 Flag pRc/CMV vector Sequence
LOCUS Stat3_Flag_pRc/C 7969 bp DNA circular SYN 13-MAY-2021
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS Stat3 Flag pRc/CMV.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7969)
AUTHORS Horvath CM, Wen Z, Darnell JE Jr
TITLE A STAT protein domain that determines DNA sequence recognition
suggests a novel DNA-binding domain.
JOURNAL Genes Dev 1995 Apr 15;9(8):984-94.
PUBMED 7774815
REFERENCE 2 (bases 1 to 7969)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 7969)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev";
date: "1995-04-15"; volume: "9(8)"; pages: "984-94"
COMMENT SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7969
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 103..120
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 237..258
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 273..303
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 311..327
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 335..351
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
polyA_signal complement(382..516)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
CDS complement(693..1484)
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
promoter complement(1551..1880)
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin complement(1908..2421)
/direction=LEFT
/label=M13 ori
/note="M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
polyA_signal complement(2467..2691)
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
promoter 2717..2735
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
CDS complement(2744..2767)
/label=FLAG
/note="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
CDS complement(2774..5083)
/gene="Stat3"
/label=Stat3
/note="Signal transducer and activator of transcription 3
from Mus musculus. Accession#: P42227"
primer_bind complement(5175..5191)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(5282..5300)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter complement(5345..5548)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
enhancer complement(5549..5928)
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
primer_bind 6100..6119
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
promoter 6194..6298
/label=AmpR promoter
CDS 6299..7156
/label=AmpR
/note="beta-lactamase"
rep_origin 7330..7918
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
This page is informational only.