HLTV-hTRIM21 vector (V000027)

MSDS

Basic Vector Information

- - Vector Name:
  - HLTV-hTRIM21
- - Antibiotic Resistance:
  - Ampicillin
- - Length:
  - 4456 bp
- - Type:
  - Bacterial Expression
- - Replication origin:
  - ori
- - Promoter:
  - T7
- - Cloning Method:
  - Restriction Enzyme
- - 5' Primer:
  - TAA TAC GAC TCA CTA TAG GG

HLTV-hTRIM21 vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

HLTV-hTRIM21 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       HLTV-hTRIM21.        4456 bp DNA     circular SYN 09-SEP-2021
DEFINITION  Purification of His-Lipoyl-hTRIM21 protein from bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    HLTV-hTRIM21.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Clift D, McEwan WA, Labzin LI, Konieczny V, Mogessie B, James LC, 
            Schuh M
  TITLE     A Method for the Acute and Rapid Degradation of Endogenous Proteins.
  JOURNAL   Cell. 2017 Nov 13. pii: S0092-8674(17)31255-2. doi: 
            10.1016/j.cell.2017.10.033.
  PUBMED    29153837
REFERENCE   2  (bases 1 to 4456)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4456)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2017 
            Nov 13. pii: S0092-8674(17)31255-2. doi: 
            10.1016/j.cell.2017.10.033."
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..56
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     290..307
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        392..410
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             442..464
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             475..492
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             769..789
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     CDS             796..2220
                     /gene="TRIM21"
                     /label=TRIM21
                     /note="E3 ubiquitin-protein ligase TRIM21 from Homo
                     sapiens. Accession#: P19474"
     terminator      2243..2290
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      2387..2842
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2868..2972
                     /label=AmpR promoter
     CDS             2973..3830
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4004..4456
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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