-3.6kb human IFN-gamma luc vector (V000180)

Price Information

Cat No. Plasmid Name Availability Add to cart
V000180 -3.6kb human IFN-gamma luc In stock (lyophilized plasmid)

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Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

-3.6kb human IFN-gamma luc is a mammalian expression vector with luciferase

      • Vector Name:
      • -3.6kb human IFN-gamma luc
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 8832 bp
      • Type:
      • Mammalian Expression, Luciferase
      • Copy Number:
      • High Copy
      • Cloning Method:
      • Restriction Enzyme
      • 5' Primer:
      • RVprimer3
      • 3' Primer:
      • LucNrev
      • Growth Strain(s):
      • DH5alpha
      • Growth Temperature:
      • 37℃

-3.6kb human IFN-gamma luc vector Vector Map

-3.6kb human IFN-gamma luc8832 bp40080012001600200024002800320036004000440048005200560060006400680072007600800084008800luciferaseSV40 poly(A) signalL4440oriAmpRAmpR promoterf1 orisynthetic polyadenylation signalpause siteKozak sequence

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

References

  • Gonsky R, Deem RL, Bream JH, Lee DH, Young HA, Targan SR. Mucosa-specific targets for regulation of IFN-gamma expression: lamina propria T cells use different cis-elements than peripheral blood T cells to regulate transactivation of IFN-gamma expression. J Immunol. 2000 Feb 1;164(3):1399-407.

-3.6kb human IFN-gamma luc vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                8832 bp ds-DNA     circular SYN 12-JUL-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    -3.6kb human IFN-gamma luc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8832)
  AUTHORS   Gonsky R, Deem RL, Bream JH, Lee DH, Young HA, Targan SR
  TITLE     Mucosa-specific targets for regulation of IFN-gamma expression: 
            lamina propria T cells use different cis-elements than peripheral 
            blood T cells to regulate transactivation of IFN-gamma expression.
  JOURNAL   J Immunol. 2000 Feb 1. 164(3):1399-407.
  PUBMED    10640755
REFERENCE   2  (bases 1 to 8832)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Immunol. 
            2000 Feb 1. 164(3):1399-407."
FEATURES             Location/Qualifiers
     source          1..8832
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1..1653
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(67..85)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    1694..1815
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1705..1724)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1759..1778
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2063..2080)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2234..2822)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2314..2333)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2993..3853)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3616..3635
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3854..3958)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3985..4440
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4072..4091)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4282..4303
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    4571..4619
                     /note="synthetic polyadenylation signal"
     misc_feature    4633..4724
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4673..4692
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      join(8827..8832,1..4)
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"