CaV1.2 vector (V000220)

Basic Vector Information

      • Vector Name:
      • CaV1.2
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 11709 bp
      • Type:
      • Mammalian Expression
      • Replication origin:
      • ori
      • Promoter:
      • CMV
      • Cloning Method:
      • Restriction Enzyme
      • 5' Primer:
      • T7
      • 3' Primer:
      • BGH-rev

CaV1.2 vector Vector Map

CaV1.211709 bp50010001500200025003000350040004500500055006000650070007500800085009000950010000105001100011500CAP binding sitelac promoterlac operatorM13 revSV40 poly(A) signalBSDEM7 promoterSV40 promoterpBABE 3'f1 oribGH poly(A) signal6xHisV5 tagCacna1cT7 promoterCMV promoterCMV enhancerpRS-markerAmpR promoterAmpRoriL4440

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

CaV1.2 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       CaV1.2.       11709 bp DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11709)
  AUTHORS   Helton TD, Xu W, Lipscombe D
  TITLE     Neuronal L-type calcium channels open quickly and are inhibited 
  JOURNAL   J Neurosci. 2005 Nov 2. 25(44):10247-51.
  PUBMED    16267232
REFERENCE   2  (bases 1 to 11709)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 11709)
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2005 Nov 2. 25(44):10247-51."
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    complement(258..391)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(552..947)
                     /note="blasticidin S deaminase"
     promoter        complement(966..1013)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     promoter        complement(1069..1279)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     primer_bind     1303..1323
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(1332..1760)
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1442..1463)
                     /note="F1 origin, forward primer"
     primer_bind     1654..1673
                     /note="F1 origin, reverse primer"
     polyA_signal    complement(1806..2030)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(2059..2076)
                     /note="6xHis affinity tag"
     CDS             complement(2086..2127)
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             complement(2332..8748)
                     /note="Voltage-dependent L-type calcium channel subunit
                     alpha-1C from Mus musculus. Accession#: Q01815"
     promoter        complement(8892..8910)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(8955..9158)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
     enhancer        complement(9159..9538)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     9710..9729
                     /note="pRS vectors, use to sequence yeast selectable
     promoter        9804..9908
                     /label=AmpR promoter
     CDS             9909..10766
     rep_origin      10940..11528
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
     primer_bind     11682..11699
                     /note="L4440 vector, forward primer"

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