Basic Vector Information
Beta-arrestin2 GFP WT vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
Beta-arrestin2 GFP WT vector Sequence
LOCUS Beta-arrestin2_G 5954 bp DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS Beta-arrestin2 GFP WT. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5954) AUTHORS Shenoy SK, Lefkowitz RJ TITLE Receptor-specific ubiquitination of beta-arrestin directs assembly and targeting of seven-transmembrane receptor signalosomes. JOURNAL J Biol Chem. 2005 Apr 15;280(15):15315-24. Epub 2005 Feb 7. PUBMED 15699045 REFERENCE 2 (bases 1 to 5954) TITLE Direct Submission REFERENCE 3 (bases 1 to 5954) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J Biol Chem."; date: "2005-04-15"; volume: "280(15)"; pages: "15315-24. Epub 2005 Feb 7" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..5954 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 1..304 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 305..508 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" CDS 583..1812 /gene="Arrb2" /label=Arrb2 /note="Beta-arrestin-2 from Rattus norvegicus. Accession#: P29067" CDS 1840..2556 /label=EGFP /note="enhanced GFP" polyA_signal 2682..2803 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(2810..3265) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 3292..3396 /label=AmpR promoter promoter 3398..3755 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 3790..4581 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" primer_bind complement(4772..4791) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4816..4863 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 5192..5780 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" promoter 5895..5954 /label=CMV /note="Human cytomegalovirus immediate early enhancer/promoter"
This page is informational only.