Beta-arrestin2 GFP WT vector (V000378)

Basic Vector Information

      • Vector Name:
      • Beta-arrestin2 GFP WT
      • Antibiotic Resistance:
      • Kanamycin
      • Length:
      • 5954 bp
      • Type:
      • Mammalian Expression
      • Copy Number:
      • High Copy
      • Promoter:
      • CMV
      • Cloning Method:
      • Restriction Enzyme
      • 3' Primer:
      • EGFP-N

Beta-arrestin2 GFP WT vector Vector Map

Beta-arrestin2 GFP WT5954 bp60012001800240030003600420048005400CMV enhancerCMV promoterArrb2EGFPSV40 poly(A) signalf1 oriAmpR promoterSV40 promoterNeoR/KanRTK-pA-RHSV TK poly(A) signaloriCMV

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

Beta-arrestin2 GFP WT vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Beta-arrestin2_G        5954 bp DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    Beta-arrestin2 GFP WT.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Shenoy SK, Lefkowitz RJ
  TITLE     Receptor-specific ubiquitination of beta-arrestin directs assembly 
            and targeting of seven-transmembrane receptor signalosomes.
  JOURNAL   J Biol Chem. 2005 Apr 15;280(15):15315-24. Epub 2005 Feb 7.
  PUBMED    15699045
REFERENCE   2  (bases 1 to 5954)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5954)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol
            Chem."; date: "2005-04-15"; volume: "280(15)"; pages: "15315-24.
            Epub 2005 Feb 7"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             583..1812
                     /gene="Arrb2"
                     /label=Arrb2
                     /note="Beta-arrestin-2 from Rattus norvegicus. Accession#: 
                     P29067"
     CDS             1840..2556
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    2682..2803
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2810..3265)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3292..3396
                     /label=AmpR promoter
     promoter        3398..3755
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3790..4581
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     primer_bind     complement(4772..4791)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4816..4863
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5192..5780
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        5895..5954
                     /label=CMV
                     /note="Human cytomegalovirus immediate early
                     enhancer/promoter"

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