Basic Vector Information
| Basic Vector Information | |||
|---|---|---|---|
| Vector Name | pTracer-CMV2 | Antibiotic Resistance | Ampicillin |
| Length | 6210 bp | Type | Mammalian Expression Vectors |
| Selection Marker | Zeocin | Promoter | CMV |
pTracer-CMV2 vector Vector Map
Plasmid Resuspension protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pTracer-CMV2 vector Sequence
LOCUS Exported 6210 bp ds-DNA circular SYN 13-JAN-2022
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6210)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..6210
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 235..614
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal 1028..1139
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
promoter 1309..2487
/note="EF-1-alpha promoter"
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 1540..2478
/note="EF-1-alpha intron A"
/note="intron upstream of the start codon of human
EF-1-alpha"
promoter 2504..2522
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
promoter 2611..2658
/note="EM7 promoter"
/note="synthetic bacterial promoter "
CDS 3376..3753
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/note="BleoR"
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MDAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWG
REFALRDPAGNCVHFVAEEQD"
polyA_signal 3883..4004
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
primer_bind complement(4053..4069)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 4077..4093
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4101..4131)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 4146..4167
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4455..5043)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5214..6074)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6075..6179)
/gene="bla"
/note="AmpR promoter"
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