Basic Vector Information
AAV zinc finger donor vector for genomic targeting
- Vector Name:
- AAV-CAGGS-EGFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9809 bp
- Type:
- TALEN-targeted gene manipulation vectors
- Replication origin:
- ori
- Selection Marker:
- EGFP
- Promoter:
- CAG
AAV-CAGGS-EGFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
AAV-CAGGS-EGFP vector Sequence
LOCUS 40924_130 9809 bp DNA circular SYN 13-JAN-2022
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9809)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 9809)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9809
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 33..836
/label=HA-L
/note="left homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
misc_feature 843..868
/label=SA
/note="splice acceptor site"
CDS 892..945
/label=T2A
/note="2A peptide from Thosea asigna virus capsid protein"
CDS 955..1551
/label=PuroR
/note="puromycin N-acetyltransferase"
polyA_signal 1592..1816
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
enhancer 1822..2201
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 2203..2480
/note="chicken beta-actin promoter"
intron 2482..3499
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
CDS 3561..4277
/label=EGFP
/note="enhanced GFP"
polyA_signal 4435..4490
/label=beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(4851..4867)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(4875..4891)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4899..4929)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(4944..4965)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature 5039..5875
/label=HA-R
/note="right homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
promoter complement(5916..5934)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(5941..5957)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
CDS 6095..6394
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
CDS complement(7797..8654)
/label=AmpR
/note="beta-lactamase"
rep_origin 8778..9366
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 9654..9675
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 9690..9720
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9728..9744
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 9752..9768
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 9789..9807
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
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