CFP-C1-PLCdelta-PH vector (V000461)

Basic Vector Information

      • Vector Name:
      • CFP-C1-PLCdelta-PH
      • Antibiotic Resistance:
      • Kanamycin
      • Length:
      • 5229 bp
      • Type:
      • Mammalian Expression
      • Selection Marker:
      • Neomycin (select with G418)
      • Cloning Method:
      • Restriction Enzyme

CFP-C1-PLCdelta-PH vector Vector Map

CFP-C1-PLCdelta-PH5229 bp6001200180024003000360042004800CMV enhancerCMV promoterCFPMCSSV40 poly(A) signalf1 oriAmpR promoterSV40 promoterNeoR/KanRTK-pA-RHSV TK poly(A) signalori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

CFP-C1-PLCdelta-PH vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                5229 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Botelho RJ, Teruel M, Dierckman R, Anderson R, Wells A, York JD, 
            Meyer T, Grinstein S
  TITLE     Localized biphasic changes in phosphatidylinositol-4,5-bisphosphate 
            at sites of phagocytosis.
  JOURNAL   J Cell Biol. 2000 Dec 25. 151(7):1353-68.
  PUBMED    11134066
REFERENCE   2  (bases 1 to 5229)
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2000 Dec 25. 151(7):1353-68."
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
     primer_bind     519..539
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             598..1314
                     /product="cyan variant of GFP"
                     /note="mammalian codon-optimized"
     primer_bind     complement(643..664)
                     /note="EGFP, reverse primer"
     primer_bind     complement(904..923)
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1251..1272
                     /note="EGFP, forward primer"
     misc_feature    1315..1371
                     /note="multiple cloning site"
     polyA_signal    2017..2138
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2054..2073)
                     /note="SV40 polyA, reverse primer"
     primer_bind     2108..2127
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2145..2600)
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2282..2303)
                     /note="F1 origin, forward primer"
     primer_bind     2494..2513
                     /note="F1 origin, reverse primer"
     promoter        2627..2731
                     /label=AmpR promoter
     promoter        2733..3090
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(2756..2776)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2941..3076
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3003..3022
                     /note="SV40 promoter/origin, forward primer"
     CDS             3125..3919
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="confers resistance to neomycin, kanamycin, and G418 
     primer_bind     complement(3179..3198)
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3789..3808
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4107..4126)
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4151..4198
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4527..5115
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
     primer_bind     5016..5035
                     /note="pBR322 origin, forward primer"

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