1.3xWT HBV-Luciferase vector (V000707)

Price Information

Cat No. Plasmid Name Availability Add to cart
V000707 1.3xWT HBV-Luciferase In stock, 1 week for quality controls

Buy one, get one free!

Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.

Basic Vector Information

Vector Name:
1.3xWT HBV-Luciferase
Antibiotic Resistance:
Ampicillin
Length:
6656 bp
Type:
Mammalian Expression, Luciferase
Replication origin:
ori
Promoter:
SP6

1.3xWT HBV-Luciferase vector Vector Map

1.3xWT HBV-Luciferase6656 bp3006009001200150018002100240027003000330036003900420045004800510054005700600063006600Protein XluciferaseProtein XSP6 promoterM13 revlac operatorlac promoterCAP binding siteL4440oriAmpRAmpR promoterpBRforEcopGEX 3'pRS-markerM13 fwdT7 promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

1.3xWT HBV-Luciferase vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V000707                 6656 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V000707
VERSION     V000707
KEYWORDS    1.3xWT HBV-Luciferase
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6656)
  AUTHORS   Doitsh G, Shaul Y
  TITLE     Enhancer I predominance in hepatitis B virus gene expression.
  JOURNAL   Mol Cell Biol. 2004 Feb;24(4):1799-808.
   PUBMED   14749394
REFERENCE   2  (bases 1 to 6656)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6656)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell
            Biol."; date: "2004-02"; volume: "24(4)"; pages: "1799-808"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             355..816
                     /gene="X"
                     /label="Protein X"
                     /note="Protein X from Hepatitis B virus genotype A2 subtype
                     adw2 (strain Rutter 1979). Accession#: P69713"
     CDS             969..2618
                     /label="luciferase"
                     /note="firefly luciferase"
     CDS             3317..3778
                     /gene="X"
                     /label="Protein X"
                     /note="Protein X from Hepatitis B virus genotype A2 subtype
                     adw2 (strain Rutter 1979). Accession#: P69713"
     promoter        complement(3981..3999)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4017..4033)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4041..4057)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4065..4095)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4110..4131)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     primer_bind     complement(4248..4265)
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4419..5007)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5181..6038)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6039..6143)
                     /label="AmpR promoter"
     primer_bind     6211..6229
                     /label="pBRforEco"
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
     primer_bind     complement(6267..6289)
                     /label="pGEX 3'"
                     /note="pGEX vectors, reverse primer"
     primer_bind     6389..6408
                     /label="pRS-marker"
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     primer_bind     6617..6633
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6640..6656
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"