MXS_BidirectionalCAG vector (V000726)

MSDS

Basic Vector Information

- - Vector Name:
  - MXS_BidirectionalCAG
- - Antibiotic Resistance:
  - Ampicillin
- - Length:
  - 6221 bp
- - Type:
  - Mammalian Expression, Synthetic Biology
- - Replication origin:
  - ori
- - Copy Number:
  - High Copy
- - Promoter:
  - CAG
- - Cloning Method:
  - Restriction Enzyme
- - 5' Primer:
  - TTACCGCCTTTGAGTGAG
- - 3' Primer:
  - TTGTCTCATGAGCGGATAC

MXS_BidirectionalCAG vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

MXS_BidirectionalCAG vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_2169        6221 bp DNA     circular SYN 13-MAY-2021
DEFINITION  Bidirectional CAG promoter (compatible with the MXS Chaining Kit).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     Bidirectional Promoter Engineering for Single Cell MicroRNA Sensors 
            in Embryonic Stem Cells.
  JOURNAL   PLoS One. 2016 May 6;11(5):e0155177. doi: 
            10.1371/journal.pone.0155177. eCollection 2016.
  PUBMED    27152616
REFERENCE   2  (bases 1 to 6221)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6221)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-05-6"; pages: "
            10.1371/journal.pone.0155177. eCollection 2016"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          complement(101..1117)
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     promoter        complement(1118..1394)
                     /label=chicken beta-actin promoter
     enhancer        1396..1775
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        complement(1783..2162)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        2170..2549
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        complement(2559..2936)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2946..3222
                     /label=chicken beta-actin promoter
     intron          3223..4239
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     promoter        4329..4433
                     /label=AmpR promoter
     CDS             4434..5291
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5465..6053
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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