MXS_BidirectionalCAG vector (V000726)

Basic Vector Information

      • Vector Name:
      • MXS_BidirectionalCAG
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 6221 bp
      • Type:
      • Mammalian Expression, Synthetic Biology
      • Copy Number:
      • High Copy
      • Cloning Method:
      • Restriction Enzyme
      • 5' Primer:
      • TTACCGCCTTTGAGTGAG
      • 3' Primer:
      • TTGTCTCATGAGCGGATAC

MXS_BidirectionalCAG vector Vector Map

MXS_BidirectionalCAG6221 bp30060090012001500180021002400270030003300360039004200450048005100540057006000chimeric intronchicken beta-actin promoterCMV enhancerCMV enhancerCMV enhancerCMV enhancerchicken beta-actin promoterchimeric intronAmpR promoterAmpRori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

MXS_BidirectionalCAG vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                6221 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Bidirectional CAG promoter (compatible with the MXS Chaining Kit).
ACCESSION   .
VERSION     .
KEYWORDS    MXS_BidirectionalCAG
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     Bidirectional Promoter Engineering for Single Cell MicroRNA Sensors 
            in Embryonic Stem Cells.
  JOURNAL   PLoS One. 2016 May 6;11(5):e0155177. doi: 
            10.1371/journal.pone.0155177. eCollection 2016.
  PUBMED    27152616
REFERENCE   2  (bases 1 to 6221)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-05-6"; volume: "11(5):e0155177. doi"; pages: " 
            10.1371/journal.pone.0155177. eCollection 2016"
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     intron          101..1117
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     promoter        complement(1118..1394)
                     /label=chicken beta-actin promoter
     enhancer        1396..1775
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        1783..2162
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        2170..2549
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        2557..2936
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2946..3222
                     /label=chicken beta-actin promoter
     intron          3223..4239
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     promoter        4329..4433
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4434..5294
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4652..4671)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5465..6053
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5954..5973
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"

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