Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V000765 | pLenti-CMV-MCS-GFP-SV-puro | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
The pLenti-CMV-MCS-GFP-SV-puro is a third generation lentiviral vector expressing GFP and puromycin.
- Vector Name:
- pLenti-CMV-MCS-GFP-SV-puro
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8455 bp
- Type:
- Lentiviral
- Replication origin:
- ori
- Selection Marker:
- Puromycin
- Promoter:
- RSV
- Cloning Method:
- Restriction Enzyme
- 5' Primer:
- GAGCACCCAGTCCGCCCTG
pLenti-CMV-MCS-GFP-SV-puro vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
References
- Witwicka H, Hwang SY, Reyes-Gutierrez P, Jia H, Odgren PE, Donahue LR, Birnbaum MJ, Odgren PR. Studies of OC-STAMP in Osteoclast Fusion: A New Knockout Mouse Model, Rescue of Cell Fusion, and Transmembrane Topology. PLoS One. 2015 Jun 4;10(6):e0128275. doi: 10.1371/journal.pone.0128275. PMID: 26042409; PMCID: PMC4456411.
pLenti-CMV-MCS-GFP-SV-puro vector Sequence
LOCUS pLenti-CMV-MCS-G 8455 bp DNA circular SYN 13-MAY-2021
DEFINITION Third generation lentiviral vector expressing GFP and puromycin.
ACCESSION .
VERSION .
KEYWORDS pLenti-CMV-MCS-GFP-SV-puro.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8455)
AUTHORS Witwicka H, Hwang SY, Reyes-Gutierrez P, Jia H, Odgren PE, Donahue
LR, Birnbaum MJ, Odgren PR
TITLE Studies of OC-STAMP in Osteoclast Fusion: A New Knockout Mouse
Model, Rescue of Cell Fusion, and Transmembrane Topology.
JOURNAL PLoS One. 2015 Jun 4;10(6):e0128275. doi:
10.1371/journal.pone.0128275. eCollection 2015.
PUBMED 26042409
REFERENCE 2 (bases 1 to 8455)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 8455)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
date: "2015-06-4"; pages: "
10.1371/journal.pone.0128275. eCollection 2015"
COMMENT SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..8455
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 113..134
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 149..179
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 187..203
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 211..227
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 248..266
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 294..520
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 521..701
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 748..873
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1366..1599
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1784..1828
/label=gp41 peptide
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 1977..2018
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
misc_feature 2095..2211
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 2234..2537
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2538..2741
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2738..2762
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 2818..3534
/label=EGFP
/note="enhanced GFP"
promoter 3587..3916
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 3926..4522
/label=PuroR
/note="puromycin N-acetyltransferase"
misc_feature 4592..5180
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
LTR 5267..5500
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 5572..5706
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin 5733..5868
/label=SV40 ori
/note="SV40 origin of replication"
promoter complement(5889..5907)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(5917..5933)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 6075..6530
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 6556..6660
/label=AmpR promoter
CDS 6661..7518
/label=AmpR
/note="beta-lactamase"
rep_origin 7692..8280
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8434..8451
/label=L4440
/note="L4440 vector, forward primer"