Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V000780 | pMuLE Lenti Dest iRFP | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
- Vector Name:
- pMuLE Lenti Dest iRFP
- Antibiotic Resistance:
- Chloramphenicol, Ampicillin
- Length:
- 9377 bp
- Type:
- Mammalian Expression, Lentiviral ; MuLE Gateway De
- Replication origin:
- ori
- Selection Marker:
- iRFP
- Copy Number:
- High Copy
- Promoter:
- mPGK
pMuLE Lenti Dest iRFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMuLE Lenti Dest iRFP vector Sequence
LOCUS pMuLE_Lenti_Dest 9377 bp DNA circular SYN 13-MAY-2021
DEFINITION MuLE (Multiple Lentiviral Expression) Destination vector for use
with pMuLE Entry vectors. Co-expresses iRFP under the control of a
PGK promoter. Compatible with MultiSite Gateway cloning.
ACCESSION .
VERSION .
KEYWORDS pMuLE Lenti Dest iRFP.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9377)
AUTHORS Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T,
Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK,
Bode-Lesniewska B, Wild PJ, Frew IJ
TITLE A versatile modular vector system for rapid combinatorial mammalian
genetics.
JOURNAL J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
PUBMED 25751063
REFERENCE 2 (bases 1 to 9377)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 9377)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J Clin
Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9377
/mol_type="other DNA"
/organism="synthetic DNA construct"
LTR 94..327
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 399..533
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin 539..674
/label=SV40 ori
/note="SV40 origin of replication"
promoter complement(707..725)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(735..751)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 896..1351
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1380..1484
/label=AmpR promoter
CDS 1485..2342
/label=AmpR
/note="beta-lactamase"
rep_origin 2516..3104
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3258..3275
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 3392..3413
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 3428..3458
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3466..3482
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 3490..3506
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 3527..3545
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 3573..3800
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 3801..3981
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 4025..4150
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 4643..4876
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 5060..5104
/label=gp41 peptide
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 5253..5294
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
misc_feature 5365..5482
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind 5537..5661
/label=attR1
/note="recombination site for the Gateway(R) LR reaction"
promoter 5686..5716
/label=lac UV5 promoter
/note="E. coli lac promoter with an 'up' mutation"
CDS 5770..6426
/label=CmR
/note="chloramphenicol acetyltransferase"
CDS 6771..7073
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
protein_bind complement(7117..7241)
/label=attR2
/note="recombination site for the Gateway(R) LR reaction"
misc_feature 7272..7860
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
promoter 7874..8373
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
CDS 8417..9364
/label=iRFP713
/note="phytochrome-based near-infrared fluorescent protein,
originally termed iRFP (Filonov et al., 2011)"