Basic Vector Information
- Vector Name:
- S-HBsAg
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6118 bp
- Type:
- Mammalian Expression
- Replication origin:
- ori
- Selection Marker:
- Neomycin (select with G418)
S-HBsAg vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
S-HBsAg vector Sequence
LOCUS 40924_48733 6118 bp DNA circular SYN 13-MAY-2021 DEFINITION Expresses HBV Small envelope glycoprotein in mammalian cells. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6118) AUTHORS Wang H, Ryu WS TITLE Hepatitis B virus polymerase blocks pattern recognition receptor signaling via interaction with DDX3: implications for immune evasion. JOURNAL PLoS Pathog. 2010 Jul 15;6(7):e1000986. doi: 10.1371/journal.ppat.1000986. PUBMED 20657822 REFERENCE 2 (bases 1 to 6118) TITLE Direct Submission REFERENCE 3 (bases 1 to 6118) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Pathog."; date: "2010-07-15"; pages: " 10.1371/journal.ppat.1000986" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..6118 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 956..1633 /codon_start=1 /label=HBsAg (S) /note="major/small (S) envelope protein of hepatitis B virus" /translation="MENITSGFLGPLLVLQAGFFLLTRILTIPQSLDSWWTSLNFLGGT TVCLGQNSQSPTSNHSPTSCPPTCPGYRWMCLRRFIIFLFILLLCLIFLLVLLDYQGML PVCPLIPGSSTTSTGPCRTCMTTAQGTSMYPSCCCTKPSDGNCTCIPIPSSWAFGKFLW EWASARFSWLSLLVPFVQWFVGLSPTVWLSVIWMMWYWGPSLYSILSPFLPLLPIFFCL WVYI" promoter complement(1671..1689) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" polyA_signal 1715..1939 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 1985..2413 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 2427..2756 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 2823..3614 /codon_start=1 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 3791..3924 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3961..3977) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(3985..4001) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4009..4039) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(4054..4075) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(4192..4209) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4363..4951) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5125..5982) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(5983..6087) /label=AmpR promoter
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