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pMIND vector (V000961)

Basic Vector Information

Vector Name:
pMIND
Antibiotic Resistance:
Kanamycin
Length:
6778 bp
Type:
Bacterial Expression
Replication origin:
ori
Selection Marker:
Hygromycin
Copy Number:
Low Copy
Cloning Method:
Restriction Enzyme

pMIND vector Vector Map

pMIND6778 bp3006009001200150018002100240027003000330036003900420045004800510054005700600063006600M13 revhygM13 fwdNeoR/KanRoriL4440CAP binding sitelac promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMIND vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pMIND.        6778 bp DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pMIND.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   Blokpoel MC, Murphy HN, O'Toole R, Wiles S, Runn ES, Stewart GR, 
            Young DB, Robertson BD
  TITLE     Tetracycline-inducible gene regulation in mycobacteria.
  JOURNAL   Nucleic Acids Res. 2005  . 33(2):e22.
  PUBMED    15687380
REFERENCE   2  (bases 1 to 6778)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6778)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2005  . 33(2):e22."
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..35
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             908..1903
                     /gene="hyg"
                     /label=hyg
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces 
                     hygroscopicus. Accession#: P09979"
     primer_bind     complement(1916..1932)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4745..5536
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      5823..6411
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6565..6582
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6699..6720
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6735..6765
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"

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