IKKalpha (S176E,S180E)-pcw107-V5 vector (V000986)

Basic Vector Information

      • Vector Name:
      • IKKalpha (S176E,S180E)-pcw107-V5
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 9880 bp
      • Type:
      • Lentiviral
      • Selection Marker:
      • Puromycin
      • Promoter:
      • PGK
      • Cloning Method:
      • Gateway Cloning
      • 5' Primer:
      • hPGK-F
      • 3' Primer:
      • WPRE-R

IKKalpha (S176E,S180E)-pcw107-V5 vector Vector Map

IKKalpha (S176E,S180E)-pcw107-V59880 bp40080012001600200024002800320036004000440048005200560060006400680072007600800084008800920096005' LTR (truncated)HIV-1 PsiRREgp41 peptideSV40 promoterPuroRcPPT/CTShPGK promoterattB1CHUKattB2V5 tagWPRE3' LTR (Delta-U3)SV40 poly(A) signalSV40 oriT7 promoterM13 fwdf1 oriAmpR promoterAmpRoriL4440CAP binding sitelac promoterlac operatorM13 revT3 promoterRSV promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

IKKalpha (S176E,S180E)-pcw107-V5 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       IKKalpha_(S176E,        9880 bp DNA     circular SYN 13-MAY-2021
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    IKKalpha (S176E,S180E)-pcw107-V5.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9880)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 9880)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9880)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci
            Signal."; date: "2014-12-23"; pages: "
            10.1126/scisignal.aaa1877"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /label=gp41 peptide
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et 
                     al., 2013)"
     promoter        1539..1749
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     CDS             1789..2385
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     misc_feature    2434..2551
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2606..3109
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     protein_bind    3130..3154
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3172..5406
                     /gene="CHUK"
                     /label=CHUK
                     /note="Inhibitor of nuclear factor kappa-B kinase subunit
                     alpha from Homo sapiens. Accession#: O15111"
     protein_bind    complement(5411..5431)
                     /label=attB2
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     CDS             5440..5481
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5506..6094
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6166..6399
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6477..6611
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6638..6773
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6794..6812)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6822..6838)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      6980..7435
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7461..7565
                     /label=AmpR promoter
     CDS             7566..8423
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      8597..9185
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9339..9356
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9473..9494
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9509..9539
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9547..9563
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9571..9587
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9608..9626
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9654..9880
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"

This page is informational only.