Basic Vector Information
- Vector Name:
- Matrix-roGFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6929 bp
- Type:
- Mammalian Expression, Adenoviral
- Replication origin:
- ori
- Copy Number:
- High Copy
- Promoter:
- CMV
- Cloning Method:
- Restriction Enzyme
- 5' Primer:
- 5'GGCGTGTACGGTGGGAGGTCTATA-3'
- 3' Primer:
- 5'-TTAAAAAACCTCCCCACCTCCCCC-3'
Matrix-roGFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
Matrix-roGFP vector Sequence
LOCUS Matrix-roGFP. 6929 bp DNA circular SYN 13-MAY-2021
DEFINITION Expresses thiol redox-sensitive ratiometric sensor roGFP in the
mitochondrial matrix of mammalian cells.
ACCESSION .
VERSION .
KEYWORDS Matrix-roGFP.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6929)
AUTHORS Waypa GB, Marks JD, Guzy R, Mungai PT, Schriewer J, Dokic D,
Schumacker PT
TITLE Hypoxia triggers subcellular compartmental redox signaling in
vascular smooth muscle cells.
JOURNAL Circ Res. 2010 Feb 19;106(3):526-35. doi:
10.1161/CIRCRESAHA.109.206334. Epub 2009 Dec 17.
PUBMED 20019331
REFERENCE 2 (bases 1 to 6929)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 6929)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1161/CIRCRESAHA.109.206334"; journalName: "Circ Res"; date:
"2010-02-19- 19"; volume: "106"; issue: "3"; pages: "526-35"
COMMENT SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6929
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 12..315
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 316..519
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 600..1316
/label=EGFP
/note="enhanced GFP"
polyA_signal complement(1360..1494)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1637..1659)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS 2062..2481
/gene="IX"
/label=IX
/note="Hexon-interlacing protein from Human adenovirus C
serotype 5. Accession#: P03281"
CDS complement(2547..3881)
/gene="IVa2"
/label=IVa2
/note="Packaging protein 1 from Human adenovirus C serotype
5. Accession#: P03271"
rep_origin complement(4734..5322)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5496..6353)
/label=AmpR
/note="beta-lactamase"
promoter complement(6354..6458)
/label=AmpR promoter
primer_bind 6526..6544
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
repeat_region 6572..6674
/label=ITR
/note="inverted terminal repeat of human adenovirus
serotype 5"
misc_signal 6762..6912
/label=Ad5 Psi
/note="packaging signal for adenovirus serotype 5"
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