Mfn2-YFP vector (V001384)

MSDS

Basic Vector Information

Vector Name:: Mfn2-YFP

Antibiotic Resistance:: Kanamycin

Length:: 6984 bp

Type:: Mammalian Expression

Replication origin:: ori

Selection Marker:: Neomycin (select with G418)

Copy Number:: High Copy

Cloning Method:: Restriction Enzyme

5' Primer:: AGGTCTATATAAGCAGAGC

Mfn2-YFP vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

Mfn2-YFP vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Mfn2-YFP.        6984 bp DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    Mfn2-YFP.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6984)
  AUTHORS   Karbowski M, Lee YJ, Gaume B, Jeong SY, Frank S, Nechushtan A, 
            Santel A, Fuller M, Smith CL, Youle RJ
  TITLE     Spatial and temporal association of Bax with mitochondrial fission 
            sites, Drp1, and Mfn2 during apoptosis.
  JOURNAL   J Cell Biol. 2002 Dec 23. 159(6):931-8.
  PUBMED    12499352
REFERENCE   2  (bases 1 to 6984)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6984)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2002 Dec 23. 159(6):931-8."
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        68..371
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        372..575
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             645..2915
                     /gene="MFN2"
                     /label=MFN2
                     /note="Mitofusin-2 from Homo sapiens. Accession#: O95140"
     CDS             2937..3653
                     /label=EYFP
                     /note="enhanced YFP"
     polyA_signal    3779..3900
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3907..4362)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4389..4493
                     /label=AmpR promoter
     promoter        4495..4852
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4887..5678
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     primer_bind     complement(5869..5888)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5913..5960
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6289..6877
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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