Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V001433 | T7opt in pCAGGS | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
T7opt in pCAGGS vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
T7opt in pCAGGS vector Sequence
LOCUS T7opt_in_pCAGGS. 7385 bp DNA circular SYN 17-JUN-2021
DEFINITION Human codon-optimized T7 polymerase.
ACCESSION .
VERSION .
KEYWORDS T7opt in pCAGGS.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7385)
AUTHORS Yun T, Park A, Hill TE, Pernet O, Beaty SM, Juelich TL, Smith JK,
Zhang L, Wang YE, Vigant F, Gao J, Wu P, Lee B, Freiberg AN
TITLE Efficient reverse genetics reveals genetic determinants of budding
and fusogenic differences between Nipah and Hendra viruses and
enables real-time monitoring of viral spread in small animal models
of henipavirus infection.
JOURNAL J Virol. 2015 Jan 15;89(2):1242-53. doi: 10.1128/JVI.02583-14. Epub
2014 Nov 12.
PUBMED 25392218
REFERENCE 2 (bases 1 to 7385)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 7385)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1128/JVI.02583-14"; journalName: "J Virol"; date: "2015-01-15-
15"; volume: "89"; issue: "2"; pages: "1242-53"
COMMENT SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7385
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 63..366
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 368..645
/label=chicken beta-actin promoter
intron 646..1662
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 1670..1689
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
CDS 1723..4371
/note="T7 RNA polymerase from Escherichia phage T7.
Accession#: P00573"
primer_bind complement(4389..4408)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_signal 4454..4509
/label=beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(4508..4527)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(4870..4886)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(4894..4910)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4918..4948)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(4963..4984)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5042..5238
/label=SV40 promoter
/note="SV40 early promoter"
polyA_signal 5244..5378
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5446..5463)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5617..6205)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6379..7236)
/label=AmpR
/note="beta-lactamase"
promoter complement(7237..7341)
/label=AmpR promoter
promoter 7369..7385
/label=CAG
/note="CMV early enhancer fused to modified chicken
beta-actin promoter"